HSD17B3 Protein (Myc-DYKDDDDK Tag)
Quick Overview for HSD17B3 Protein (Myc-DYKDDDDK Tag) (ABIN2714229)
Target
See all HSD17B3 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This HSD17B3 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human 17-beta-HSD3 / HSD17B3 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Hydroxysteroid (17-Beta) Dehydrogenase 3 (HSD17B3) (AA 1-310) protein (His tag)
custom-made HSD17B3 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Hydroxysteroid (17-Beta) Dehydrogenase 3 (HSD17B3) (AA 1-305) protein (His tag)custom-made HSD17B3 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Hydroxysteroid (17-Beta) Dehydrogenase 3 (HSD17B3) (AA 67-178) protein (His-IF2DI Tag)HSD17B3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Hydroxysteroid (17-Beta) Dehydrogenase 3 (HSD17B3) (AA 1-310) protein (GST tag)HSD17B3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- HSD17B3 (Hydroxysteroid (17-Beta) Dehydrogenase 3 (HSD17B3))
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Alternative Name
- 17-beta-Hsd3,hsd17b3
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Background
- This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia.
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Molecular Weight
- 34.3 kDa
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NCBI Accession
- NP_000188
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Pathways
- Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis
Target
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