beta Actin Protein (His tag)
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- Target See all beta Actin (ACTB) Proteins
- beta Actin (ACTB) (Actin, beta (ACTB))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This beta Actin protein is labelled with His tag.
- Application
- Antibody Production (AbP), Standard (STD)
- Characteristics
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- Recombinant human Actin beta / ACTB protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Purity
- > 95 % as determined by SDS-PAGE and Coomassie blue staining
- Endotoxin Level
- < 0.1 EU per μg protein as determined by LAL test
- Top Product
- Discover our top product ACTB Protein
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Actin, beta (ACTB) (AA 1-375) protein (His tag)
custom-made ACTB Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Actin, beta (ACTB) (AA 1-375) protein (His tag)custom-made ACTB Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Actin, beta (ACTB) (AA 1-375) protein (GST tag)ACTB Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Actin, beta (ACTB) (AA 14-167) protein (His tag)ACTB Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
Actin, beta (ACTB) (AA 102-375) protein (His tag)ACTB Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
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- Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays - Comment
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The tag is located at the C-terminal.
- Restrictions
- For Research Use only
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- Concentration
- 50 μg/mL
- Buffer
- 10 mM Tris-HCl, 0.1 % TritonX-100, 2 mM DTT, pH 8.0. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Target
- beta Actin (ACTB) (Actin, beta (ACTB))
- Alternative Name
- Actin Beta,actb (ACTB Products)
- Background
- This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
- Molecular Weight
- 42.8 kDa
- NCBI Accession
- NP_001092
- Pathways
- Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
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