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Actin, beta (ACTB) protein (His tag) Protein

ACTB Origin: Human Source: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, Func, PI, STD
Catalog No. ABIN2714358
$396.00
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10 μg
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  • Target
    beta Actin (ACTB)
    Protein Type
    Recombinant
    Origin
    Human
    Source
    Escherichia coli (E. coli)
    • 25
    • 4
    • 4
    • 1
    • 1
    Purification tag / Conjugate
    This beta Actin protein is labelled with His tag.
    Application
    Antibody Production (AbP), Functional Studies (Func), Protein Interaction (PI), Standard (STD)
    Specificity
    Optimal preservation of protein structure, post-translational modifications and functions.
    Characteristics
    • Recombinant human Actin beta / ACTB protein expressed in E. coli.
    • Produced with end-sequenced ORF clone
    Purity
    > 95 % as determined by SDS-PAGE and Coomassie blue staining
    Endotoxin Level
    < 0.1 EU per μg protein as determined by LAL test
  • Application Notes
    Recombinant human proteins can be used for:
    Native antigens for optimized antibody production
    Positive controls in ELISA and other antibody assays
    Protein-protein interaction
    In vitro biochemical assays and cell-based functional assays
    Comment

    The tag is located at the C-terminal.

    Restrictions
    For Research Use only
  • Concentration
    > 50 μg/mL
    Buffer
    10 mM Tris-HCl, 0.1 % TritonX-100, 2 mM DTT, pH 8.0. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
  • Target
    beta Actin (ACTB)
    Synonyms
    BRWS1, PS1TP5BP1, ACTA1, Actx, E430023M04Rik, beta-actin, ps1tp5bp1, act-2, actb, B-actin, LOC100174883, LOC100304816, A, ACT5C, Ac5C, Act, Act-5C, Act5, Act5c, Actin, Actin/BAP47, Actin5C, BAP47, Bap47, CG4027, Dmel\\CG4027, M32055, T11, act, act 5C, act5C, actin, actin5C, anon-EST:fe2D2, beta-actin/Bap47, cyt5C, l(1)G0009, l(1)G0010, l(1)G0025, l(1)G0079, l(1)G0117, l(1)G0177, l(1)G0245, l(1)G0330, l(1)G0420, l(1)G0486, Bact, ACT-5, ACTB, B-ACTZF, actba, bact, bactin1, bactzf, wu:fd18f05, Beta-actin, actin beta, actin, beta, cytoplasmic actin OlCA1, beta-actin, Actin 5C, actin gamma 1, actin, beta 1, actin, beta L homeolog, beta actin, actin, cytoplasmic 1, ACTB, Actb, actb, LOC100174883, LOC100304816, Act5C, ACTG1, actb1, actb.L, LOC100136352, LOC109073280
    Background
    This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins.
    Molecular Weight
    42.8 kDa
    NCBI Accession
    NP_001092
    Pathways
    Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction
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