alpha 2 Macroglobulin Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2714610

This Recombinant alpha 2 Macroglobulin protein is produced in HEK-293 Cells.

Quick Overview for alpha 2 Macroglobulin Protein (Myc-DYKDDDDK Tag) (ABIN2714610)

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This alpha 2 Macroglobulin protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human Alpha-2-macroglobulin / A2M protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: alpha 2 Macroglobulin (A2M) (alpha-2-Macroglobulin (A2M))

Alternative Name: alpha-2-Macroglobulin

Background: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. [UniProtKB/Swiss-Prot Function]

Molecular Weight: 160.8 kDa

NCBI Accession: NP_000005

Pathways: Lipid Metabolism