DAZL Protein (Myc-DYKDDDDK Tag)
Quick Overview for DAZL Protein (Myc-DYKDDDDK Tag) (ABIN2719233)
Target
See all DAZL ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This DAZL protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human DAZL protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Deleted in Azoospermia-Like (DAZL) (AA 1-295) protein (His tag)
custom-made DAZL Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Deleted in Azoospermia-Like (DAZL) (AA 1-298) protein (His tag)custom-made DAZL Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Deleted in Azoospermia-Like (DAZL) (AA 1-295) protein (GST tag)DAZL Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- DAZL (Deleted in Azoospermia-Like (DAZL))
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Alternative Name
- Dazl
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Background
- The DAZ (Deleted in AZoospermia) gene family encodes potential RNA binding proteins that are expressed in prenatal and postnatal germ cells of males and females. The protein encoded by this gene is localized to the nucleus and cytoplasm of fetal germ cells and to the cytoplasm of developing oocytes. In the testis, this protein is localized to the nucleus of spermatogonia but relocates to the cytoplasm during meiosis where it persists in spermatids and spermatozoa. Transposition and amplification of this autosomal gene during primate evolution gave rise to the DAZ gene cluster on the Y chromosome. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males. Two transcript variants encoding different isoforms have been found for this gene.
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Molecular Weight
- 33 kDa
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NCBI Accession
- NP_001342
Target
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