DPH1 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2719693

This Recombinant DPH1 protein is expressed in HEK-293 Cells.

Quick Overview for DPH1 Protein (Myc-DYKDDDDK Tag) (ABIN2719693)

Target: DPH1 (DPH1 Homolog (DPH1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This DPH1 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human DPH1 / OVAC1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: DPH1 (DPH1 Homolog (DPH1))

Alternative Name: Dph1,ovac1

Background: The protein encoded by this gene is an enzyme involved in the biosynthesis of diphthamide, a modified histidine found only in elongation factor-2 (EEF2). Diphthamide residues in EEF2 are targeted for ADP-ribosylation by diphtheria toxin and Pseudomonas exotoxin A. Defects in this gene have been associated with both ovarian cancer and autosomal recessive intellectual disability with short stature, craniofacial, and ectodermal anomalies.

Molecular Weight: 48.6 kDa

NCBI Accession: NP_001374