EEF1G Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2719947

This Recombinant EEF1G protein is produced in HEK-293 Cells. There is 1 publication available.

Quick Overview for EEF1G Protein (Myc-DYKDDDDK Tag) (ABIN2719947)

Target: EEF1G (Eukaryotic Translation Elongation Factor 1 gamma (EEF1G))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This EEF1G protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human EEF1G protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Li, Wei, Rawle, Qin, Wang, Soares, Jin, Sivakumaran, Lin, Spann, Abbott, Harrich: "Specific Interaction between eEF1A and HIV RT Is Critical for HIV-1 Reverse Transcription and a Potential Anti-HIV Target." in: PLoS pathogens, Vol. 11, Issue 12, pp. e1005289, (2015) (PubMed).

Target Details

Target: EEF1G (Eukaryotic Translation Elongation Factor 1 gamma (EEF1G))

Alternative Name: Eef1g

Background: This gene encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases.

Molecular Weight: 49.9 kDa

NCBI Accession: NP_001395