EWSR1 Protein (Transcript Variant EWS) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2720598

This Recombinant EWSR1 protein is produced in HEK-293 Cells. There is 1 publication available.

Quick Overview for EWSR1 Protein (Transcript Variant EWS) (Myc-DYKDDDDK Tag) (ABIN2720598)

Target: EWSR1 (Ewing Sarcoma Breakpoint Region 1 (EWSR1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant EWS

Purification tag / Conjugate: This EWSR1 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human EWSR1 (transcript variant EWS) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Altmeyer, Neelsen, Teloni, Pozdnyakova, Pellegrino, Grøfte, Rask, Streicher, Jungmichel, Nielsen, Lukas: "Liquid demixing of intrinsically disordered proteins is seeded by poly(ADP-ribose)." in: Nature communications, Vol. 6, pp. 8088, (2015) (PubMed).

Target Details

Target: EWSR1 (Ewing Sarcoma Breakpoint Region 1 (EWSR1))

Alternative Name: Ewsr1

Background: This gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t(1122)(q24q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14.

Molecular Weight: 68.3 kDa

NCBI Accession: NP_005234