Quick Overview for GGCT Protein (His tag) (ABIN2721860)
Target
See all GGCT ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This GGCT protein is labelled with His tag.
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Characteristics
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- Recombinant human GGCT protein expressed in E. coli.
- Produced with end-sequenced ORF clone
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Endotoxin Level
- < 0.1 EU per μg protein as determined by LAL test
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gamma-Glutamylcyclotransferase (GGCT) (AA 1-188) protein (His tag)
custom-made GGCT Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
gamma-Glutamylcyclotransferase (GGCT) (AA 1-188) protein (His tag)custom-made GGCT Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
gamma-Glutamylcyclotransferase (GGCT) (AA 1-188), (full length) protein (His-SUMO Tag)GGCT Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
gamma-Glutamylcyclotransferase (GGCT) protein (Myc-DYKDDDDK Tag)GGCT Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 20 mM Tris-HCl, pH 8.0. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- GGCT (gamma-Glutamylcyclotransferase (GGCT))
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Alternative Name
- Ggct
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Background
- The protein encoded by this gene catalyzes the formation of 5-oxoproline from gamma-glutamyl dipeptides, the penultimate step in glutathione catabolism, and may play a critical role in glutathione homeostasis. The encoded protein may also play a role in cell proliferation, and the expression of this gene is a potential marker for cancer. Pseudogenes of this gene are located on the long arm of chromosome 5 and the short arm of chromosomes 2 and 20. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
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Molecular Weight
- 23.2 kDa
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NCBI Accession
- NP_076956
Target
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