GLUD1 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2721997

This Recombinant GLUD1 protein is expressed in HEK-293 Cells.

Quick Overview for GLUD1 Protein (Myc-DYKDDDDK Tag) (ABIN2721997)

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This GLUD1 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human Glutamate dehydrogenase 1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: GLUD1 (Glutamate Dehydrogenase 1 (GLUD1))

Alternative Name: Glutamate Dehydrogenase 1

Background: This gene encodes glutamate dehydrogenase, which is a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid-induced insulin secretion. It is allosterically activated by ADP and inhibited by GTP and ATP. Activating mutations in this gene are a common cause of congenital hyperinsulinism. Alternative splicing of this gene results in multiple transcript variants. The related glutamate dehydrogenase 2 gene on the human X-chromosome originated from this gene via retrotransposition and encodes a soluble form of glutamate dehydrogenase. Related pseudogenes have been identified on chromosomes 10, 18 and X.

Molecular Weight: 56 kDa

NCBI Accession: NP_005262

Pathways: Positive Regulation of Peptide Hormone Secretion, Warburg Effect