HSP70 1A Protein (His tag)

Catalog No. ABIN2722556

Recombinant HSP70 1A protein expressed in Insect cells (Sf9).

Quick Overview for HSP70 1A Protein (His tag) (ABIN2722556)

Target: HSP70 1A (HSPA1A) (Heat Shock 70kDa Protein 1A (HSPA1A))

Protein Type: Recombinant

Origin: Human

Source: Insect cells (Sf9)

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This HSP70 1A protein is labelled with His tag.

Characteristics:

• Recombinant human Heat shock 70 kDa protein 1A (HSPA1A), full length, with C-terminal polyhistidine tag, expressed in sf9 cells. (full length, C-term polyhistidine tag) protein expressed in Sf9 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 50 mM Tris-HCl pH 8.0, 150 mM NaCl, 20 % glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: HSP70 1A (HSPA1A) (Heat Shock 70kDa Protein 1A (HSPA1A))

Background: This intronless gene encodes a 70 kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

Molecular Weight: 70 kDa

NCBI Accession: NP_005336

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process