HERC6 Protein (Myc-DYKDDDDK Tag)
Quick Overview for HERC6 Protein (Myc-DYKDDDDK Tag) (ABIN2722636)
Target
See all HERC6 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This HERC6 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human HERC6 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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HECT and RLD Domain Containing E3 Ubiquitin Protein Ligase Family Member 6 (HERC6) (AA 1-1022) protein (His tag)
custom-made HERC6 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
HECT and RLD Domain Containing E3 Ubiquitin Protein Ligase Family Member 6 (HERC6) (AA 1-1003) protein (His tag)custom-made HERC6 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- HERC6 (HECT and RLD Domain Containing E3 Ubiquitin Protein Ligase Family Member 6 (HERC6))
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Alternative Name
- Herc6
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Background
- HERC6 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]).[supplied by OMIM, Mar 2008]
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Molecular Weight
- 114.9 kDa
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NCBI Accession
- NP_060382
Target
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