INS-IGF2 Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2723491

This Recombinant INS-IGF2 protein is expressed in HEK-293 Cells.

Quick Overview for INS-IGF2 Protein (Transcript Variant 2) (Myc-DYKDDDDK Tag) (ABIN2723491)

Target: INS-IGF2 (INS-IGF2 Readthrough (INS-IGF2))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 2

Purification tag / Conjugate: This INS-IGF2 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human INS-IGF2 readthrough transcript (INS-IGF2), transcript variant 2 (transcript variant 2) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: INS-IGF2 (INS-IGF2 Readthrough (INS-IGF2))

Alternative Name: Ins-Igf2 Readthrough Transcript (Ins-Igf2)

Background: This locus includes two alternatively spliced read-through transcript variants which align to the INS gene in the 5' region and to the IGF2 gene in the 3' region. One transcript is predicted to encode a protein which shares the N-terminus with the INS protein but has a distinct and longer C-terminus, whereas the other transcript is a candidate for nonsense-mediated decay (NMD). The transcripts are imprinted and are paternally expressed in the limb and eye.

Molecular Weight: 21.4 kDa

NCBI Accession: NP_001035835

Pathways: Peptide Hormone Metabolism