IL-33 Protein (Transcript Variant 1)
Quick Overview for IL-33 Protein (Transcript Variant 1) (ABIN2723707)
Target
See all IL-33 (IL33) ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 1
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Specificity
- Optimal preservation of protein structure, post-translational modifications and functions.
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Characteristics
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- Recombinant human Interleukin-33 (IL33) (transcript variant 1) protein expressed in E. coli.
- Produced with end-sequenced ORF clone
- Tested for bioactivity.
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Endotoxin Level
- Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).
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Biological Activity Comment
- ED50 was determined by the dose-dependent stimulation of the proliferation of murine D10S cells is less than or equal to 0.05 ng/ml, corresponding to a specific activity of > 2 x 10^7units/mg.
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Interleukin 33 (IL33) (AA 112-270) protein
IL33 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 33 (IL33) (AA 1-266) protein (His tag)custom-made IL33 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Interleukin 33 (IL33) (AA 1-270) protein (His tag)custom-made IL33 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Interleukin 33 (IL33) (AA 109-270) (Active) protein (His tag)IL33 Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Interleukin 33 (IL33) (AA 109-264) protein (His tag)IL33 Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 33 (IL33) (AA 109-270) protein (His tag)IL33 Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 33 (IL33) protein (His tag)IL33 Origin: Rhesus Monkey Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by reducing SDS-PAGE.
Interleukin 33 (IL33) (AA 171-269) protein (GST tag)IL33 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays
Protein-protein interaction
In vitro biochemical assays and cell-based functional assays -
Restrictions
- For Research Use only
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Buffer
- Lyophilized from a 0.2 μM filtered solution of 20 mM phosphate buffer,100 mM NaCl, pH 7.2
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Handling Advice
- Resuspend the protein in the desired concentration in proper buffer
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- IL-33 (IL33) (Interleukin 33 (IL33))
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Alternative Name
- Interleukin-33 (Il33)
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Background
- The protein encoded by this gene is a cytokine that binds to the IL1RL1/ST2 receptor. The encoded protein is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. Several transcript variants encoding different isoforms have been found for this gene.
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Molecular Weight
- 17.9 kDa
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NCBI Accession
- NP_254274
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Pathways
- Production of Molecular Mediator of Immune Response
Target
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