Lactate Dehydrogenase A Protein (LDHA) (Transcript Variant 2) (His tag)

Catalog No. ABIN2724572

Recombinant Lactate Dehydrogenase A protein expressed in Escherichia coli (E. coli).

Quick Overview for Lactate Dehydrogenase A Protein (LDHA) (Transcript Variant 2) (His tag) (ABIN2724572)

Target: Lactate Dehydrogenase A (LDHA)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Antibody Production (AbP), Standard (STD)

Purity: > 95 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 2

Purification tag / Conjugate: This Lactate Dehydrogenase A protein is labelled with His tag.

Characteristics:

• Recombinant human LDHA (transcript variant 2) protein expressed in E. coli.
• Produced with end-sequenced ORF clone

Endotoxin Level: < 0.1 EU per μg protein as determined by LAL test

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 20 mM Tris-HCl, 100 mM NaCl, 50 % Glycerol, pH 8.0. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: Lactate Dehydrogenase A (LDHA)

Alternative Name: Ldha

Background: The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene.

Molecular Weight: 38.8 kDa

NCBI Accession: NP_001128711

Pathways: Warburg Effect