MARCH2 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2725491

This Recombinant MARCH2 protein is produced in HEK-293 Cells.

Quick Overview for MARCH2 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2725491)

Target: MARCH2 (Membrane-Associated Ring Finger (C3HC4) 2, E3 Ubiquitin Protein Ligase (MARCH2))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 1

Purification tag / Conjugate: This MARCH2 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human MARCH2 / RNF172 (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: MARCH2 (Membrane-Associated Ring Finger (C3HC4) 2, E3 Ubiquitin Protein Ligase (MARCH2))

Alternative Name: March2,rnf172

Background: MARCH2 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH2 reduces surface accumulation of several glycoproteins and appears to regulate early endosome-to-trans-Golgi network (TGN) trafficking (Bartee et al., 2004 [PubMed 14722266] Nakamura et al., 2005 [PubMed 15689499]).[supplied by OMIM, Mar 2010]

Molecular Weight: 26.8 kDa

NCBI Accession: NP_057580