MARCKS Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2725496

Recombinant MARCKS protein expressed in HEK-293 Cells and has been mentioned in 1 publication.

Quick Overview for MARCKS Protein (Myc-DYKDDDDK Tag) (ABIN2725496)

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This MARCKS protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human MARCKS protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Park, Kang, Shin, Seo, Kim, Suh, Bae, Shin: "Identification of novel phosphatidic acid-binding proteins in the rat brain." in: Neuroscience letters, Vol. 595, pp. 108-13, (2015) (PubMed).

Target Details

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Alternative Name: Marcks

Background: MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein. [UniProtKB/Swiss-Prot Function]

Molecular Weight: 31.4 kDa

NCBI Accession: NP_002347