MGEA5 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)
(1 reference)Quick Overview for MGEA5 Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2725936)
Target
See all MGEA5 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 1
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Purification tag / Conjugate
- This MGEA5 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human MGEA5 (transcript variant 1) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Meningioma Expressed Antigen 5 (Hyaluronidase) (MGEA5) (AA 1-916) protein (His tag)
custom-made MGEA5 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Meningioma Expressed Antigen 5 (Hyaluronidase) (MGEA5) (AA 1-916) protein (His tag)custom-made MGEA5 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Meningioma Expressed Antigen 5 (Hyaluronidase) (MGEA5) (AA 1-209) protein (His-IF2DI Tag)MGEA5 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Meningioma Expressed Antigen 5 (Hyaluronidase) (MGEA5) (AA 1-916) protein (GST tag)MGEA5 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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: "Thiamet-G-mediated inhibition of O-GlcNAcase sensitizes human leukemia cells to microtubule-stabilizing agent paclitaxel." in: Biochemical and biophysical research communications, Vol. 453, Issue 3, pp. 392-7, (2014) (PubMed).
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: "Thiamet-G-mediated inhibition of O-GlcNAcase sensitizes human leukemia cells to microtubule-stabilizing agent paclitaxel." in: Biochemical and biophysical research communications, Vol. 453, Issue 3, pp. 392-7, (2014) (PubMed).
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- MGEA5 (Meningioma Expressed Antigen 5 (Hyaluronidase) (MGEA5))
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Alternative Name
- Mgea5
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Background
- The dynamic modification of cytoplasmic and nuclear proteins by O-linked N-acetylglucosamine (O-GlcNAc) addition and removal on serine and threonine residues is catalyzed by OGT (MIM 300255), which adds O-GlcNAc, and MGEA5, a glycosidase that removes O-GlcNAc modifications (Gao et al., 2001 [PubMed 11148210]).[supplied by OMIM, Mar 2008].
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Molecular Weight
- 102.7 kDa
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NCBI Accession
- NP_036347
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Pathways
- Positive Regulation of Peptide Hormone Secretion, Regulation of Carbohydrate Metabolic Process
Target
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