Nebulette Protein (NEBL) (Transcript Variant 2) (Myc-DYKDDDDK Tag)
Quick Overview for Nebulette Protein (NEBL) (Transcript Variant 2) (Myc-DYKDDDDK Tag) (ABIN2727047)
Target
See all Nebulette (NEBL) ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 2
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Purification tag / Conjugate
- This Nebulette protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human Nebulette / NEBL (transcript variant 2) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Nebulette (NEBL) (AA 1-270) protein (His tag)
custom-made NEBL Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Nebulette (NEBL) (AA 1-452) protein (His tag)custom-made NEBL Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Nebulette (NEBL) (AA 1-1014) protein (His tag)custom-made NEBL Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Nebulette (NEBL)
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Alternative Name
- Nebulette,nebl
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Background
- This gene encodes a nebulin like protein that is abundantly expressed in cardiac muscle. The encoded protein binds actin and interacts with thin filaments and Z-line associated proteins in striated muscle. This protein may be involved in cardiac myofibril assembly. A shorter isoform of this protein termed LIM nebulette is expressed in non-muscle cells and may function as a component of focal adhesion complexes. Alternate splicing results in multiple transcript variants.
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Molecular Weight
- 31 kDa
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NCBI Accession
- NP_998734
Target
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