LCP1 Protein (Myc-DYKDDDDK Tag)

Catalog No. ABIN2729119

This Recombinant LCP1 protein is expressed in HEK-293 Cells and has been mentioned in 1 publication.

Quick Overview for LCP1 Protein (Myc-DYKDDDDK Tag) (ABIN2729119)

Target: LCP1 (Lymphocyte Cytosolic Protein 1 (LCP1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Purification tag / Conjugate: This LCP1 protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human Plastin 2 / LCP1 protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

References

Dubey, Singh, Awasthi, Nagarkoti, Kumar, Ali, Chandra, Kumar, Barthwal, Jagavelu, Sánchez-Gómez, Lamas, Dikshit: "L-Plastin S-glutathionylation promotes reduced binding to β-actin and affects neutrophil functions." in: Free radical biology & medicine, Vol. 86, pp. 1-15, (2015) (PubMed).

Target Details

Target: LCP1 (Lymphocyte Cytosolic Protein 1 (LCP1))

Alternative Name: Plastin 2,lcp1

Background: Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). However, L-plastin has been found in many types of malignant human cells of non-hemopoietic origin suggesting that its expression is induced accompanying tumorigenesis in solid tissues.

Molecular Weight: 70.1 kDa

NCBI Accession: NP_002289