PPM1A Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag)

Catalog No. ABIN2729790

This Recombinant PPM1A protein is produced in HEK-293 Cells.

Quick Overview for PPM1A Protein (Transcript Variant 1) (Myc-DYKDDDDK Tag) (ABIN2729790)

Target: PPM1A (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1A (PPM1A))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Antibody Production (AbP), Standard (STD)

Purity: > 80 % as determined by SDS-PAGE and Coomassie blue staining

Product Details

Protein Characteristics: Transcript Variant 1

Purification tag / Conjugate: This PPM1A protein is labelled with Myc-DYKDDDDK Tag.

Characteristics:

• Recombinant human Protein phosphatase 1A / PPM1A (transcript variant 1) protein expressed in HEK293 cells.
• Produced with end-sequenced ORF clone

Application Details

Application Notes: Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays

Comment:

The tag is located at the C-terminal.

Restrictions: For Research Use only

Handling

Concentration: 50 μg/mL

Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.

Storage: -80 °C

Storage Comment: Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.

Target Details

Target: PPM1A (Protein Phosphatase, Mg2+/Mn2+ Dependent, 1A (PPM1A))

Alternative Name: Protein Phosphatase 1a,ppm1a

Background: The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described.

Molecular Weight: 42.3 kDa

NCBI Accession: NP_066283