Ribonuclease H1 Protein (RNASEH1) (Myc-DYKDDDDK Tag)
Quick Overview for Ribonuclease H1 Protein (RNASEH1) (Myc-DYKDDDDK Tag) (ABIN2730997)
Target
See all Ribonuclease H1 (RNASEH1) ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This Ribonuclease H1 protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human RNASEH1 protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Ribonuclease H1 (RNASEH1) (AA 1-285) protein (His tag)
custom-made RNASEH1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ribonuclease H1 (RNASEH1) (AA 1-286) protein (His tag)custom-made RNASEH1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ribonuclease H1 (RNASEH1) (AA 1-286) protein (GST tag)RNASEH1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Ribonuclease H1 (RNASEH1) (AA 189-286) protein (GST tag)RNASEH1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- Ribonuclease H1 (RNASEH1)
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Alternative Name
- Rnaseh1
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Background
- This gene encodes an endonuclease that specifically degrades the RNA of RNA-DNA hybrids and is necessary for DNA replication and repair. This enzyme is present in both mitochondria and nuclei, which are resulted from translation of a single mRNA with two in-frame initiation start codons. The use of the first start codon produces the mitochondrial isoform and the use of the second start codon produces the nuclear isoform. The production of the mitochondrial isoform is modulated by an upstream open reading frame (uORF) which overlaps the first initiation start codon in human. An alternately spliced transcript variant has been found which encodes a shorter isoform. This gene has three pseudogenes two of them are at different locations of chromosome 17 and one of them is on chromosome 1q32.2.
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Molecular Weight
- 31.9 kDa
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NCBI Accession
- NP_002927
Target
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