SEC31A Protein (Transcript Variant 3) (Myc-DYKDDDDK Tag)
Quick Overview for SEC31A Protein (Transcript Variant 3) (Myc-DYKDDDDK Tag) (ABIN2731578)
Target
See all SEC31A ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- Transcript Variant 3
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Purification tag / Conjugate
- This SEC31A protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human SEC31A (transcript variant 3) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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SEC31 Homolog A (SEC31A) (AA 1-1220) protein (His tag)
custom-made SEC31A Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
SEC31 Homolog A (SEC31A) (AA 1-1230) protein (His tag)custom-made SEC31A Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
SEC31 Homolog A (SEC31A) (AA 1-509) protein (GST tag)SEC31A Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- SEC31A (SEC31 Homolog A (SEC31A))
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Alternative Name
- Sec31a
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Background
- The protein encoded by this gene shares similarity with the yeast Sec31 protein, and is a component of the outer layer of the coat protein complex II (COPII). The encoded protein is involved in vesicle budding from the endoplasmic reticulum (ER) and contains multiple WD repeats near the N-terminus and a proline-rich region in the C-terminal half. It associates with the protein encoded by the SEC13 homolog, nuclear pore and COPII coat complex component (SEC13), and is required for ER-Golgi transport. Monoubiquitylation of this protein by CUL3-KLHL12 was found to regulate the size of COPII coats to accommodate unusually shaped cargo. Alternative splicing results in multiple transcript variants encoding different isoforms.
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Molecular Weight
- 131.4 kDa
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NCBI Accession
- NP_001070676
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Pathways
- ER-Nucleus Signaling
Target
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