STIP1 Protein (DYKDDDDK Tag)
Quick Overview for STIP1 Protein (DYKDDDDK Tag) (ABIN2732856)
Target
See all STIP1 ProteinsProtein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This STIP1 protein is labelled with DYKDDDDK Tag.
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Characteristics
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- Recombinant human STIP1 (full length, C-term DDK tag) protein expressed in sf9 cells.
- Produced with end-sequenced ORF clone
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Stress-Induced-phosphoprotein 1 (STIP1) (AA 1-543) protein (His tag)
custom-made STIP1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Stress-Induced-phosphoprotein 1 (STIP1) (AA 1-543) protein (His tag)custom-made STIP1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Stress-Induced-phosphoprotein 1 (STIP1) (AA 1-543) protein (GST tag)STIP1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Stress-Induced-phosphoprotein 1 (STIP1) (AA 445-543) protein (GST tag)STIP1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Stress-Induced-phosphoprotein 1 (STIP1) (AA 1-543) protein (His tag)STIP1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Stress-Induced-phosphoprotein 1 (STIP1) (AA 1-543) protein (His tag)STIP1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Stress-Induced-phosphoprotein 1 (STIP1) (AA 1-543) proteinSTIP1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 50 mM Tris-HCl, pH 8.0, 100 mM glycine, 10 % glycerol. Store at -80C. Avoid repeated freeze-thaw cycles. Stable for at least 3 months from receipt of products under proper storage and handling conditions.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- STIP1 (Stress-Induced-phosphoprotein 1 (STIP1))
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Alternative Name
- Stip1
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Background
- STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A MIM 140550) and HSP90 (see HSP90AA1 MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 [PubMed 16100115]).[supplied by OMIM, Jul 2009]
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Molecular Weight
- 62.6 kDa
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NCBI Accession
- NP_006810
Target
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