TIMELESS Protein (Myc-DYKDDDDK Tag)
Quick Overview for TIMELESS Protein (Myc-DYKDDDDK Tag) (ABIN2733738)
Target
Protein Type
Origin
Source
Application
Purity
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Purification tag / Conjugate
- This TIMELESS protein is labelled with Myc-DYKDDDDK Tag.
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Characteristics
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- Recombinant human Timeless (Tim) protein expressed in HEK293 cells.
- Produced with end-sequenced ORF clone
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Timeless Homolog (TIMELESS) (AA 1-1197) protein (His tag)
custom-made TIMELESS Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Timeless Homolog (TIMELESS) (AA 1-1208) protein (His tag)custom-made TIMELESS Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Timeless Homolog (TIMELESS) (AA 1109-1207) protein (GST tag)TIMELESS Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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Application Notes
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Recombinant human proteins can be used for:
Native antigens for optimized antibody production
Positive controls in ELISA and other antibody assays -
Comment
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The tag is located at the C-terminal.
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Restrictions
- For Research Use only
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Concentration
- 50 μg/mL
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Buffer
- 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10 % glycerol.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C. Thaw on ice, aliquot to individual single-use tubes, and then re-freeze immediately. Only 2-3 freeze thaw cycles are recommended.
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- TIMELESS (Timeless Homolog (TIMELESS))
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Alternative Name
- Timeless (Tim)
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Background
- The protein encoded by this gene is highly conserved and is involved in cell survival after damage or stress, increase in DNA polymerase epsilon activity, maintenance of telomere length, and epithelial cell morphogenesis. The encoded protein also plays a role in the circadian rhythm autoregulatory loop, interacting with the PERIOD genes (PER1, PER2, and PER3) and others to downregulate activation of PER1 by CLOCK/ARNTL. Changes in this gene or its expression may promote prostate cancer, lung cancer, breast cancer, and mental disorders.
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Molecular Weight
- 138.5 kDa
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NCBI Accession
- NP_003911
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Pathways
- Protein targeting to Nucleus, Photoperiodism
Target
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