EnhancedFasL protein (DYKDDDDK Tag)

Catalog No. ABIN2745658

This Recombinant protein is expressed in HEK-293 Cells.

Quick Overview for EnhancedFasL protein (DYKDDDDK Tag) (ABIN2745658)

Target: EnhancedFasL

Protein Type: Recombinant

Origin: Human, Mouse

Source: HEK-293 Cells

Application: Biochemical Assay (BCA), SDS-PAGE (SDS)

Product Details

Purification tag / Conjugate: DYKDDDDK Tag

Specificity: Binds to human and mouse Fas (CD95, APO-1).

Cross-Reactivity: Human, Mouse (Murine)

Components: 1 Set: 1x10μg of FasL, Soluble (human) (rec.) 4x50μg of TNF Ligands Enhancer

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Source/Host: Produced in HEK 293 cells. A FLAG®-tag is fused at the N-terminus of the extracellular domain of human FasL (aa 103-281). Concentration: FasL: 0.1mg/ml after reconstitution TNF Ligands Enhancer: 1mg/ml after reconstitution Purity: >95% (SDS-PAGE)Formulation: Lyophilized. Contains PBS. Endotoxin Content: FasL: <0.05EU/μg purified protein (LAL test, Lonza). TNF Ligands Enhancer: <0.01EU/μg purified protein (LAL test, Lonza). Reconstitution: FasL: reconstitute with 100μl sterile water. PBS containing at least 0.1% BSA should be used for further dilutions. TNF Ligands Enhancer: reconstitute each vial with 50μl sterile water. PBS containing at least 0.1% BSA should be used for further dilutions.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C.

Expiry Date: 6 months

Target Details

Target: EnhancedFasL

Background: FasL, Soluble (human) (rec.) induces apoptosis in a concentration range of <1 ng/mL in the presence of 0.1 to 1 μg/mL of TNF Ligands Enhancer . (Optimal conditions must be determined individually for each cell line tested)