EnhancedFasL protein (DYKDDDDK Tag)
Origin: Human, Mouse
Host: HEK-293 Cells
Recombinant
BCA, SDS
-
- Target
- EnhancedFasL
- Protein Type
- Recombinant
- Origin
- Human, Mouse
- Source
- HEK-293 Cells
- Purification tag / Conjugate
- DYKDDDDK Tag
- Application
- Biochemical Assay (BCA), SDS-PAGE (SDS)
- Specificity
- Binds to human and mouse Fas (CD95, APO-1).
- Cross-Reactivity
- Human, Mouse (Murine)
- Components
- 1 Set: 1x10μg of FasL, Soluble (human) (rec.) 4x50μg of TNF Ligands Enhancer
-
- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
-
Source/Host: Produced in HEK 293 cells. A FLAG®-tag is fused at the N-terminus of the extracellular domain of human FasL (aa 103-281). Concentration: FasL: 0.1mg/ml after reconstitution TNF Ligands Enhancer: 1mg/ml after reconstitution Purity: >95% (SDS-PAGE)Formulation: Lyophilized. Contains PBS. Endotoxin Content: FasL: <0.05EU/μg purified protein (LAL test, Lonza). TNF Ligands Enhancer: <0.01EU/μg purified protein (LAL test, Lonza). Reconstitution: FasL: reconstitute with 100μl sterile water. PBS containing at least 0.1% BSA should be used for further dilutions. TNF Ligands Enhancer: reconstitute each vial with 50μl sterile water. PBS containing at least 0.1% BSA should be used for further dilutions.
- Restrictions
- For Research Use only
-
- Concentration
- Lot specific
- Storage
- 4 °C,-20 °C
- Storage Comment
-
Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C. - Expiry Date
- 6 months
-
- Target
- EnhancedFasL
- Background
- FasL, Soluble (human) (rec.) induces apoptosis in a concentration range of <1 ng/mL in the presence of 0.1 to 1 μg/mL of TNF Ligands Enhancer . (Optimal conditions must be determined individually for each cell line tested)
-
You are here:
