NADK Protein (His tag)
Quick Overview for NADK Protein (His tag) (ABIN2745747)
Target
See all NADK ProteinsProtein Type
Origin
Source
Purity
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Purification tag / Conjugate
- This NADK protein is labelled with His tag.
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Purpose
- NAD Kinase (M. tuberculosis) (rec.) (His)
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Characteristics
- NAD kinase (Mycobacterium tuberculosis) is fused at the N-terminus to a His-tag.
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Biological Activity Comment
- ~1.2 U/mg protein. (Substrate: NAD (+ ATP)) ~3.8 U/mg protein. (Substrate: NAD (+ poly(P)) One unit is defined as the amount of enzyme that synthesizes 1μmol nicotinamide adenine dinucleotide phosphate (NADP) per min.
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NAD Kinase (NADK) (AA 1-439) protein (His tag)
custom-made NADK Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
NAD Kinase (NADK) (AA 1-446) protein (His tag)custom-made NADK Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
NAD Kinase (NADK) (AA 64-298) protein (His tag)NADK Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
NAD Kinase (NADK) (AA 1-110) protein (GST tag)NADK Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
NAD Kinase (NADK) (AA 1-446) protein (GST tag)NADK Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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Restrictions
- For Research Use only
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Format
- Liquid
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Reconstitution
- 0.5 mg/mL (Lot specific)
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Concentration
- Lot specific
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Buffer
- In 10 mM TRIS at pH 8.0, containing 1 mM MgCl2, 0.5 mM EDTA, 1 mM DTT and 1 mM PMSF.
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Handling Advice
- After opening, prepare aliquots and store at -80 °C.Avoid freeze/thaw cycles.
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Storage
- -20 °C,-80 °C
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Storage Comment
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Short Term Storage: -20°C
Long Term Storage: -80°C
Use & Stability: Stable for at least 6 months after receipt when stored at -80°C.
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Expiry Date
- 6 months
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- NADK (NAD Kinase (NADK))
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Alternative Name
- NAD Kinase
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Background
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Probable Inorganic Polyphosphate/ATP-NAD kinase, Poly(P)/ATP NAD Kinase 1, ppnK1, EC 2.7.1.23
NAD kinase catalyzes the transfer of a phosphate group from ATP to NAD+ to generate NADP+, which in its reduced form acts as an electron donor for biosynthetic reactions. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis. NAD kinase from Mycobacterium tuberculosis is not ATP-dependent but has a broader substrate specificity than human NAD kinase. It can economically produce NADP+ by using other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus. Was identified as a high-confidence drug target. Catalytic activity: ATP + NAD+ = ADP + NADP+
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Molecular Weight
- ~33.0kDa
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UniProt
- P0A5S6
Target
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