NADK Protein
Quick Overview for NADK Protein (ABIN2745748)
Target
See all NADK ProteinsProtein Type
Origin
Source
Purity
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Purpose
- NAD Kinase (B. subtilis) (rec.)
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Characteristics
- Full-length NAD kinase (Bacillus subtilis).
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Biological Activity Comment
- ~2.6 U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1μmol nicotinamide adenine dinucleotide phosphate (NADP) per min.
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NAD Kinase (NADK) (AA 1-439) protein (His tag)
custom-made NADK Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
NAD Kinase (NADK) (AA 1-446) protein (His tag)custom-made NADK Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
NAD Kinase (NADK) (AA 64-298) protein (His tag)NADK Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
NAD Kinase (NADK) (AA 1-110) protein (GST tag)NADK Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
NAD Kinase (NADK) (AA 1-446) protein (GST tag)NADK Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
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Restrictions
- For Research Use only
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Format
- Liquid
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Reconstitution
- Lot specific
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Concentration
- Lot specific
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Buffer
- In 50 mM TRIS-HCl, pH 7.0 containing 150 mM sodium chloride, 1 mM EDTA and 1 mM dithiothreitol.
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Handling Advice
- After opening, prepare aliquots and store at -80 °C.Avoid freeze/thaw cycles.
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Storage
- -20 °C,-80 °C
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Storage Comment
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Short Term Storage: -20°C
Long Term Storage: -80°C
Use & Stability: Stable for at least 6 months after receipt when stored at -80°C.
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Expiry Date
- 6 months
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- NADK (NAD Kinase (NADK))
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Alternative Name
- NAD Kinase
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Background
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Probable Inorganic Polyphosphate/ATP-NAD Kinase, Poly(P)/ATP NAD Kinase, ppnK, yjbN, EC 2.7.1.23
NAD kinase catalyzes the transfer of a phosphate group from ATP to NAD+ to generate NADP+, which in its reduced form acts as an electron donor for biosynthetic reactions. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis. NAD kinase from Bacillus subtilis is not ATP-dependent but has a broader substrate specificity than human NAD kinase. It can economically produce NADP+ by using other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus. Catalytic activity: ATP + NAD+ = ADP + NADP+
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Molecular Weight
- ~30.0kDa
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UniProt
- O31612
Target
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