NAD Synthetase Protein

Catalog No. ABIN2745749

Recombinant protein expressed in Escherichia coli (E. coli).

Quick Overview for NAD Synthetase Protein (ABIN2745749)

Target: NAD Synthetase

Protein Type: Recombinant

Origin: Bacillus subtilis

Source: Escherichia coli (E. coli)

Purity: >98 % (SDS-PAGE)

Product Details

Purpose: NAD Synthetase (B. subtilis) (rec.)

Characteristics: Full-length NAD synthetase (Bacillus subtilis).

Biological Activity Comment: ~0.3 U/mg protein. One unit is defined as the amount of enzyme that synthesizes 1μmol NAD per min.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: 0.5 mg/mL (Lot specific)

Concentration: Lot specific

Buffer: In 50 mM TRIS-HCl, pH 8, containing 75 mM sodium chloride, 5 % glycerol and 5 mM DTT.

Handling Advice: After opening, prepare aliquots and store at -80 °C.Avoid freeze/thaw cycles.

Storage: -20 °C,-80 °C

Storage Comment:

Short Term Storage: -20°C

Long Term Storage: -80°C

Use & Stability: Stable for at least 6 months after receipt when stored at -80°C.

Expiry Date: 6 months

Target Details

Target: NAD Synthetase

Background:

NH(3)-dependent NAD(+) Synthetase, General Stress Protein 38, GSP38, Spore Outgrowth Factor B, EC6.3.1.5

NAD synthetase is an essential enzyme involved in both the de novo biosynthesis and salvage of NAD+, catalyzing the final step of both pathways. Since NAD has a vital role in cell metabolism, the enzyme represents a valid target for the development of new antimycobacterial agents. It was shown to be induced by heat shock, salt stress, oxidative stress, glucose limitation and oxygen limitation and is involved in biological processes like stress response and sporulation resulting in formation of a cellular spore. Catalytic activity: ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+

Molecular Weight: ~30.4kDa

UniProt: P08164