VEGF Protein (AA 27-147) (His tag)

Catalog No. ABIN2870705

Product Details

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 27-147

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This VEGF protein is labelled with His tag.

Sequence: AA 27-147

Characteristics: This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 15 kDa. As a result of glycosylation, the protein migrates as 17 kDa and 20 kDa under reducing (R) condition, and 35-45 kDa under non-reducing (NR) condition (SDS-PAGE).

Purity: >95 % as determined by reduced SDS-PAGE.

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Target Details

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Alternative Name: VEGF (VEGF Products)

Background: Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, and is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. Alternatively spliced isoforms of 121,145,165,183,189 and 206 amino acids in length are expressed in humans. VEGF165 appears to be the most abundant and potent isoform, followed by VEGF121 and VEGF189. VEGF121 is the only form that lacks a basic heparinbinding region and is freely diffusible. Mouse embryos expressing only the corresponding isoform (VEGF120) do not survive to term, and show defects in skeletogenesis. Human VEGF121 shares 87 % aa sequence identity with corresponding regions of mouse and rat, 93 % with feline, equine and bovine, and 91 %, 95 % and 96 % with ovine, canine and porcine VEGF, respectively. VEGF121 induces the proliferation of lymphatic endothelial cells. The lymphangiogenesis may be promoted by upregulation of VEGF121, which may in turn act in part via induction of VEGF-C.

Molecular Weight: 14.9 kDa