VEGF Protein (AA 27-191)

Catalog No. ABIN2181903

Product Details

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 27-191

Origin: Human

Source: HEK-293 Cells

Brand: ActiveMax®

Sequence: AA 27-191

Characteristics: This protein carries no "tag". The protein has a calculated MW of 19 kDa (monomer). As a result of glycosylation, the protein migrates as 24 kDa (monomer) under reducing (R) condition, and 43-50 kDa (homodimer) under non-reducing (NR) condition (SDS-PAGE).

Purity: >98 % as determined by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1.0 EU per μg by the LAL method.

Grade: HPLC verified

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Handling Advice: Please avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: No activity loss was observed after storage at: In lyophilized state for 1 year (4 °C), After reconstitution under sterile conditions for 3 months (-70 °C).

References

Stumpf, Wimmer, Lorenz, Stieger: "Creation of different bioluminescence resonance energy transfer based biosensors with high affinity to VEGF." in: PLoS ONE, Vol. 15, Issue 3, pp. e0230344, (2020) (PubMed).

Chen, Hsueh, Lee, Tsai, Tsai, Chiang: "FGF primes angioblast formation by inducing ETV2 and LMO2 via FGFR1/BRAF/MEK/ERK." in: Cellular and molecular life sciences : CMLS, (2020) (PubMed).

Keys, Wetter, Hang, Rutschmann, Russo, Mally, Steffen, Zuppiger, Müller, Schneider, Faridmoayer, Lin, Aebi: "A biosynthetic route for polysialylating proteins in Escherichia coli." in: Metabolic engineering, Vol. 44, pp. 293-301, (2018) (PubMed).

Target Details

Target: VEGF (Vascular Endothelial Growth Factor (VEGF))

Alternative Name: VEGF (VEGF Products)

Synonyms: MVCD1 Protein, VEGF Protein, VPF Protein, Vegf Protein, Vegf120 Protein, Vegf164 Protein, Vegf188 Protein, Vpf Protein, VEGF-A Protein, VEGF164 Protein, eVEGF120 Protein, eVEGF164 Protein, vegf-a Protein, vegf Protein, vascular endothelial growth factor A Protein, vascular endothelial growth factor A L homeolog Protein, vascular endothelial growth factor Protein, vascular endothelial growth factor precursor Protein, VEGFA Protein, Vegfa Protein, vegfa.L Protein, VEGF Protein, vegf Protein

Background: VEGF165 is the most abundant splice variant of VEGF-A. VEGF165 is produced by a number of cells including endothelial cells, macrophages and T cells. VEGF165 is involved in angiogenesis, vascular endothelial cell survival, growth, migration and vascular permeability. VEGF gene expression is induced by hypoxia, inflammatory cytokines and oncogenes. VEGF165 binds to heparan sulfate and is retained on the cell surface and in the extracellular matrix. VEGF165 binds to the receptor tyrosine kinases, VEGFR1 and VEGFR2. VEGF165 is the only splice variant that binds to co-receptors NRP-1 and NRP-2 that function to enhance VEGFR2 signaling. Binding of VEGF165 to VEGFR1 and VEGFR2 leads to activation of the PI3K/AKT, p38 MAPK, FAK and paxillin. VEGF plays a key role in tumor angiogenesis in many cancers.

Molecular Weight: 19.2 kDa

NCBI Accession: NP_001165097