ARIP4 Protein (AA 1-1467) (Strep Tag)

Catalog No. ABIN3089270

Product Details

Target: ARIP4 (RAD54L2) (RAD54-Like 2 (RAD54L2))

Protein Type: Recombinant

Protein Characteristics: AA 1-1467

Origin: Human

Source: Cell-free protein synthesis (CFPS)

Purification tag / Conjugate: This ARIP4 protein is labelled with Strep Tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA

Brand: AliCE®

Sequence: MSDESASGSD PDLDPDVELE DAEEEEEEEE VAVEECDRDD EEDLLDDPSL EGMCGTEHAQ LGEDGQQPPR CTSTTSSQSE PSEQLRRHQG KNLASEDPKK KRAQKPSHMR RNIRKLLRED QLEPVTKAAQ QEELERRKRL EQQRKDYAAP IPTVPLEFLP EEIALRASDG PQLPPRVLAQ EVICLDSSSG SEDEKSSRDE VIELSSGEED TLHIVDSSES VSEDDEEEEK GGTHVNDVLN QRDALGRVLV NLNHPPEEEN VFLAPQLARA VKPHQIGGIR FLYDNLVESL ERFKTSSGFG CILAHSMGLG KTLQVISFID VLFRHTPAKT VLAIVPVNTL QNWLAEFNMW LPPPEALPAD NKPEEVQPRF FKVHILNDEH KTMASRAKVM ADWVSEGGVL LMGYEMYRLL TLKKSFATGR PKKTKKRSHP VIIDLDEEDR QQEFRREFEK ALCRPGPDVV ICDEGHRIKN CQASTSQALK NIRSRRRVVL TGYPLQNNLI EYWCMVDFVR PDFLGTRQEF SNMFERPILN GQCIDSTPQD VRLMRYRSHV LHSLLEGFVQ RRGHTVLKIH LPAKEENVIL VRLSKIQRDL YTQFMDRFRD CGSSGWLGLN PLKAFCVCCK IWNHPDVLYE ALQKESLANE QDLDVEELGS AGTSARCPPQ GTKGKGEDST LASSMGEATN SKFLQGVGFN PFQERGNNIV TYEWAKDLLT NYQTGVLENS PKMVLLFHLI EESVKLGDKI LVFSQSLSTL ALIEEFLGKR EVPCPPGTEG QGAQKWVRNI SYFRLDGSTP AFERERLINQ FNDPSNLTTW LFLLSTRAGC LGVNLIGANR VVVFDASWNP CHDAQAVCRV YRYGQKKPCY IYRLVADYTL EKKIYDRQIS KQGMSDRVVD DLNPMLNFTR KEVENLLHFV EKEPAPQVSL NVKGIKESVL QLACLKYPHL ITKEPFEHES LLLNRKDHKL TKAEKKAAKK SYEEDKRTSV PYTRPSYAQY YPASDQSLTS IPAFSQRNWQ PTLKGDEKPV ASVRPVQSTP IPMMPRHVPL GGSVSSASST NPSMNFPINY LQRAGVLVQK VVTTTDIVIP GLNSSTDVQA RINAGESIHI IRGTKGTYIR TSDGRIFAVR ATGKPKVPED GRMAASGSQG PSCESTSNGR HSASSPKAPD PEGLARPVSP DSPEIISELQ QYADVAAARE SRQSSPSTNA ALPGPPAQLM DSSAVPGTAL GTEPRLGGHC LNSSLLVTGQ PCGDRHPVLD LRGHKRKLAT PPAAQESSRR RSRKGHLPAP VQPYEHGYPV SGGFAMPPVS LNHNLTTPFT SQAGENSLFM GSTPSYYQLS NLLADARLVF PVTTDPLVPA GPVSSSSTAT SVTASNPSFM LNPSVPGILP SYSLPFSQPL LSEPRMFAPF PSPVLPSNLS RGMSIYPGYM SPHAGYPAGG LLRSQVPPFD SHEVAEVGFS SNDDEDKDDD VIEVTGK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: ARIP4 (RAD54L2) (RAD54-Like 2 (RAD54L2))

Alternative Name: RAD54L2 (RAD54L2 Products)

Background: Helicase ARIP4 (EC 3.6.4.12) (Androgen receptor-interacting protein 4) (RAD54-like protein 2),FUNCTION: DNA helicase that modulates androgen receptor (AR)-dependent transactivation in a promoter-dependent manner. Not able to remodel mononucleosomes in vitro (By similarity). {ECO:0000250}.

Molecular Weight: 162.8 kDa

UniProt: Q9Y4B4