FHOD3 Protein (AA 1-1422) (Strep Tag)

Catalog No. ABIN3092583

This Recombinant FHOD3 protein is expressed in Cell-free protein synthesis (CFPS).

Quick Overview for FHOD3 Protein (AA 1-1422) (Strep Tag) (ABIN3092583)

Target: FHOD3 (Formin Homology 2 Domain Containing 3 (FHOD3))

Protein Type: Recombinant

Origin: Human

Source: Cell-free protein synthesis (CFPS)

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1422

Purification tag / Conjugate: This FHOD3 protein is labelled with Strep Tag.

Sequence: MATLACRVQF LDDTDPFNST NFPEPSRPPL FTFREDLALG TQLAGVHRLL QAPHKLDDCT LQLSHNGAYL DLEATLAEQR DELEGFQDDA GRGKKHSIIL RTQLSVRVHA CIEKLYNSSG RDLRRALFSL KQIFQDDKDL VHEFVVAEGL TCLIKVGAEA DQNYQNYILR ALGQIMLYVD GMNGVINRNE TIQWLYTLIG SKFRLVVKTA LKLLLVFVEY SESNAPLLIQ AVTAVDTKRG VKPWSNIMEI LEEKDGVDTE LLVYAMTLVN KTLSGLPDQD TFYDVVDCLE ELGIAAVSQR HLNKKGTDLD LVEQLNIYEV ALRHEDGDET TEPPPSGCRD RRRASVCSSG GGEHRGLDRR RSRRHSVQSI KSTLSAPTSP CSQSAPSFKP NQVRDLREKY SNFGNNSYHS SRPSSGSSVP TTPTSSVSPP QEARLERSSP SGLLTSSFRQ HQESLAAERE RRRQEREERL QRIEREERNK FRYKYLEQLA AEEHEKELRS RSVSRGRADL SLDLTSPAAP ACLAPLSHSP SSSDSQEALT VSASSPGTPH HPQASAGDPE PESEAEPEAE AGAGQVADEA GQDIASAHEG AETEVEQALE QEPEERASLS EKERQNEGVN ERDNCSASSV SSSSSTLERE EKEDKLSRDR TTGLWPAGVQ DAGVNGQCGD ILTNKRFMLD MLYAHNRKSP DDEEKGDGEA GRTQQEAEAV ASLATRISTL QANSQTQDES VRRVDVGCLD NRGSVKAFAE KFNSGDLGRG SISPDAEPND KVPETAPVQP KTESDYIWDQ LMANPRELRI QDMDFTDLGE EDDIDVLDVD LGHREAPGPP PPPPPTFLGL PPPPPPPLLD SIPPPPVPGN LLVPPPPVFN APQGLGWSQV PRGQPTFTKK KKTIRLFWNE VRPFDWPCKN NRRCREFLWS KLEPIKVDTS RLEHLFESKS KELSVSKKTA ADGKRQEIIV LDSKRSNAIN IGLTVLPPPR TIKIAILNFD EYALNKEGIE KILTMIPTDE EKQKIQEAQL ANPEIPLGSA EQFLLTLSSI SELSARLHLW AFKMDYETTE KEVAEPLLDL KEGIDQLENN KTLGFILSTL LAIGNFLNGT NAKAFELSYL EKVPEVKDTV HKQSLLHHVC TMVVENFPDS SDLYSEIGAI TRSAKVDFDQ LQDNLCQMER RCKASWDHLK AIAKHEMKPV LKQRMSEFLK DCAERIIILK IVHRRIINRF HSFLLFMGHP PYAIREVNIN KFCRIISEFA LEYRTTRERV LQQKQKRANH RERNKTRGKM ITDSGKFSGS SPAPPSQPQG LSYAEDAAEH ENMKAVLKTS SPSVEDATPA LGVRTRSRAS RGSTSSWTMG TDDSPNVTDD AADEIMDRIV KSATQVPSQR VVPRERKRSR ANRKSLRRTL KSGLTPEEAR ALGLVGTSEL QL
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: FHOD3 (Formin Homology 2 Domain Containing 3 (FHOD3))

Alternative Name: FHOD3

Background: FH1/FH2 domain-containing protein 3 (Formactin-2) (Formin homolog overexpressed in spleen 2) (hFHOS2),FUNCTION: Actin-organizing protein that may cause stress fiber formation together with cell elongation (By similarity). Isoform 4 may play a role in actin filament polymerization in cardiomyocytes. {ECO:0000250, ECO:0000269|PubMed:21149568}.

Molecular Weight: 158.6 kDa

UniProt: Q2V2M9

Pathways: Regulation of Actin Filament Polymerization