MCM10 Protein (AA 1-875) (Strep Tag)

Catalog No. ABIN3093779

This Recombinant MCM10 protein is produced in Cell-free protein synthesis (CFPS).

Quick Overview for MCM10 Protein (AA 1-875) (Strep Tag) (ABIN3093779)

Target: MCM10 (Minichromosome Maintenance Deficient 10 (MCM10))

Protein Type: Recombinant

Origin: Human

Source: Cell-free protein synthesis (CFPS)

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Product Details

Protein Characteristics: AA 1-875

Purification tag / Conjugate: This MCM10 protein is labelled with Strep Tag.

Sequence: MDEEEDNLSL LTALLEENES ALDCNSEENN FLTRENGEPD AFDELFDADG DGESYTEEAD DGETGETRDE KENLATLFGD MEDLTDEEEV PASQSTENRV LPAPAPRREK TNEELQEELR NLQEQMKALQ EQLKVTTIKQ TASPARLQKS PVEKSPRPPL KERRVQRIQE STCFSAELDV PALPRTKRVA RTPKASPPDP KSSSSRMTSA PSQPLQTISR NKPSGITRGQ IVGTPGSSGE TTQPICVEAF SGLRLRRPRV SSTEMNKKMT GRKLIRLSQI KEKMAREKLE EIDWVTFGVI LKKVTPQSVN SGKTFSIWKL NDLRDLTQCV SLFLFGEVHK ALWKTEQGTV VGILNANPMK PKDGSEEVCL SIDHPQKVLI MGEALDLGTC KAKKKNGEPC TQTVNLRDCE YCQYHVQAQY KKLSAKRADL QSTFSGGRIP KKFARRGTSL KERLCQDGFY YGGVSSASYA ASIAAAVAPK KKIQTTLSNL VVKGTNLIIQ ETRQKLGIPQ KSLSCSEEFK ELMDLPTCGA RNLKQHLAKA TASGIMGSPK PAIKSISASA LLKQQKQRML EMRRRKSEEI QKRFLQSSSE VESPAVPSSS RQPPAQPPRT GSEFPRLEGA PATMTPKLGR GVLEGDDVLF YDESPPPRPK LSALAEAKKL AAITKLRAKG QVLTKTNPNS IKKKQKDPQD ILEVKERVEK NTMFSSQAED ELEPARKKRR EQLAYLESEE FQKILKAKSK HTGILKEAEA EMQERYFEPL VKKEQMEEKM RNIREVKCRV VTCKTCAYTH FKLLETCVSE QHEYHWHDGV KRFFKCPCGN RSISLDRLPN KHCSNCGLYK WERDGMLKEK TGPKIGGETL LPRGEEHAKF LNSLK
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: MCM10 (Minichromosome Maintenance Deficient 10 (MCM10))

Alternative Name: MCM10

Background: Protein MCM10 homolog (HsMCM10),FUNCTION: Acts as a replication initiation factor that brings together the MCM2-7 helicase and the DNA polymerase alpha/primase complex in order to initiate DNA replication. Additionally, plays a role in preventing DNA damage during replication. Key effector of the RBBP6 and ZBTB38-mediated regulation of DNA-replication and common fragile sites stability, acts as a direct target of transcriptional repression by ZBTB38 (PubMed:24726359). {ECO:0000269|PubMed:11095689, ECO:0000269|PubMed:15136575, ECO:0000269|PubMed:17699597, ECO:0000269|PubMed:19608746, ECO:0000269|PubMed:24726359, ECO:0000269|PubMed:32865517}.

Molecular Weight: 98.2 kDa

UniProt: Q7L590

Pathways: Mitotic G1-G1/S Phases, DNA Replication