SEC24C Protein (AA 1-1094) (Strep Tag)

Catalog No. ABIN3095305

Product Details

Target: SEC24C (SEC24 Family, Member C (SEC24C))

Protein Type: Recombinant

Protein Characteristics: AA 1-1094

Origin: Human

Source: Cell-free protein synthesis (CFPS)

Purification tag / Conjugate: This SEC24C protein is labelled with Strep Tag.

Application: Western Blotting (WB), ELISA, SDS-PAGE (SDS)

Sequence: MNVNQSVPPV PPFGQPQPIY PGYHQSSYGG QSGSTAPAIP YGAYNGPVPG YQQTPPQGMS RAPPSSGAPP ASTAQAPCGQ AAYGQFGQGD VQNGPSSTVQ MQRLPGSQPF GSPLAPVGNQ PPVLQPYGPP PTSAQVATQL SGMQISGAVA PAPPSSGLGF GPPTSLASAS GSFPNSGLYG SYPQGQAPPL SQAQGHPGIQ TPQRSAPSQA SSFTPPASGG PRLPSMTGPL LPGQSFGGPS VSQPNHVSSP PQALPPGTQM TGPLGPLPPM HSPQQPGYQP QQNGSFGPAR GPQSNYGGPY PAAPTFGSQP GPPQPLPPKR LDPDAIPSPI QVIEDDRNNR GTEPFVTGVR GQVPPLVTTN FLVKDQGNAS PRYIRCTSYN IPCTSDMAKQ AQVPLAAVIK PLARLPPEEA SPYVVDHGES GPLRCNRCKA YMCPFMQFIE GGRRFQCCFC SCINDVPPQY FQHLDHTGKR VDAYDRPELS LGSYEFLATV DYCKNNKFPS PPAFIFMIDV SYNAIRTGLV RLLCEELKSL LDFLPREGGA EESAIRVGFV TYNKVLHFYN VKSSLAQPQM MVVSDVADMF VPLLDGFLVN VNESRAVITS LLDQIPEMFA DTRETETVFV PVIQAGMEAL KAAECAGKLF LFHTSLPIAE APGKLKNRDD RKLINTDKEK TLFQPQTGAY QTLAKECVAQ GCCVDLFLFP NQYVDVATLS VVPQLTGGSV YKYASFQVEN DQERFLSDLR RDVQKVVGFD AVMRVRTSTG IRAVDFFGAF YMSNTTDVEL AGLDGDKTVT VEFKHDDRLN EESGALLQCA LLYTSCAGQR RLRIHNLALN CCTQLADLYR NCETDTLINY MAKFAYRGVL NSPVKAVRDT LITQCAQILA CYRKNCASPS SAGQLILPEC MKLLPVYLNC VLKSDVLQPG AEVTTDDRAY VRQLVTSMDV TETNVFFYPR LLPLTKSPVE STTEPPAVRA SEERLSNGDI YLLENGLNLF LWVGASVQQG VVQSLFSVSS FSQITSGLSV LPVLDNPLSK KVRGLIDSLR AQRSRYMKLT VVKQEDKMEM LFKHFLVEDK SLSGGASYVD FLCHMHKEIR QLLS
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: SEC24C (SEC24 Family, Member C (SEC24C))

Alternative Name: SEC24C (SEC24C Products)

Background: Protein transport protein Sec24C (SEC24-related protein C),FUNCTION: Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules for their transport to the Golgi complex (PubMed:10214955, PubMed:17499046, PubMed:18843296, PubMed:20427317). Plays a central role in cargo selection within the COPII complex and together with SEC24D may have a different specificity compared to SEC24A and SEC24B (PubMed:17499046, PubMed:20427317, PubMed:18843296). May more specifically package GPI-anchored proteins through the cargo receptor TMED10 (PubMed:20427317). May also be specific for IxM motif-containing cargos like the SNAREs GOSR2 and STX5 (PubMed:18843296). {ECO:0000269|PubMed:10214955, ECO:0000269|PubMed:17499046, ECO:0000269|PubMed:18843296, ECO:0000269|PubMed:20427317}.

Molecular Weight: 118.3 kDa

UniProt: P53992