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SLC15A4 Protein (AA 1-577) (Strep Tag)

Recombinant SLC15A4 protein expressed in Cell-free protein synthesis (CFPS).
Catalog No. ABIN3116562

Quick Overview for SLC15A4 Protein (AA 1-577) (Strep Tag) (ABIN3116562)

Target

SLC15A4 (Solute Carrier Family 15 (H+/Peptide Transporter), Member 4 (SLC15A4))

Protein Type

Recombinant

Origin

  • 2
  • 1
Human

Source

  • 2
  • 1
Cell-free protein synthesis (CFPS)

Application

ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Purity

approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade

custom-made
  • Protein Characteristics

    AA 1-577

    Purification tag / Conjugate

    This SLC15A4 protein is labelled with Strep Tag.

    Sequence

    MEGSGGGAGE RAPLLGARRA AAAAAAAGAF AGRRAACGAV LLTELLERAA FYGITSNLVL FLNGAPFCWE GAQASEALLL FMGLTYLGSP FGGWLADARL GRARAILLSL ALYLLGMLAF PLLAAPATRA ALCGSARLLN CTAPGPDAAA RCCSPATFAG LVLVGLGVAT VKANITPFGA DQVKDRGPEA TRRFFNWFYW SINLGAILSL GGIAYIQQNV SFVTGYAIPT VCVGLAFVVF LCGQSVFITK PPDGSAFTDM FKILTYSCCS QKRSGERQSN GEGIGVFQQS SKQSLFDSCK MSHGGPFTEE KVEDVKALVK IVPVFLALIP YWTVYFQMQT TYVLQSLHLR IPEISNITTT PHTLPAAWLT MFDAVLILLL IPLKDKLVDP ILRRHGLLPS SLKRIAVGMF FVMCSAFAAG ILESKRLNLV KEKTINQTIG NVVYHAADLS LWWQVPQYLL IGISEIFASI AGLEFAYSAA PKSMQSAIMG LFFFFSGVGS FVGSGLLALV SIKAIGWMSS HTDFGNINGC YLNYYFFLLA AIQGATLLLF LIISVKYDHH RDHQRSRANG VPTSRRA
    Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

    Characteristics

    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified in one-step affinity chromatography
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    Purification

    One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).
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  • Application Notes

    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

    Comment

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Restrictions

    For Research Use only
  • Format

    Liquid

    Buffer

    The buffer composition is at the discretion of the manufacturer.
    Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

    Handling Advice

    Avoid repeated freeze-thaw cycles.

    Storage

    -80 °C

    Storage Comment

    Store at -80°C.

    Expiry Date

    12 months
  • Target

    SLC15A4 (Solute Carrier Family 15 (H+/Peptide Transporter), Member 4 (SLC15A4))

    Alternative Name

    SLC15A4

    Background

    Solute carrier family 15 member 4 (Peptide transporter 4) (Peptide/histidine transporter 1) (hPHT1),FUNCTION: Proton-coupled amino-acid transporter that mediates the transmembrane transport of L-histidine and some di- and tripeptides from inside the lysosome to the cytosol, and plays a key role in innate immune response (PubMed:16289537, PubMed:25238095, PubMed:29224352). Able to transport a variety of di- and tripeptides, including carnosine and some peptidoglycans (PubMed:29224352, PubMed:31073693). Transporter activity is pH -dependent and maximized in the acidic lysosomal environment (By similarity). Involved in the detection of microbial pathogens by toll-like receptors (TLRs) and NOD-like receptors (NLRs), probably by mediating transport of bacterial peptidoglycans across the endolysosomal membrane: catalyzes the transport of certain bacterial peptidoglycans, such as muramyl dipeptide (MDP), the NOD2 ligand, and L-alanyl-gamma-D-glutamyl-meso-2,6-diaminoheptanedioate (tri-DAP), the NOD1 ligand (PubMed:25238095, PubMed:29224352). Required for TLR7, TLR8 and TLR9-mediated type I interferon (IFN-I) productions in plasmacytoid dendritic cells (pDCs) (PubMed:25238095). Independently of its transporter activity, also promotes the recruitment of innate immune adapter TASL to endolysosome downstream of TLR7, TLR8 and TLR9: TASL recruitment leads to the specific recruitment and activation of IRF5 (PubMed:32433612). Required for isotype class switch recombination to IgG2c isotype in response to TLR9 stimulation (By similarity). Required for mast cell secretory-granule homeostasis by limiting mast cell functions and inflammatory responses (By similarity). {ECO:0000250|UniProtKB:O09014, ECO:0000250|UniProtKB:Q91W98, ECO:0000269|PubMed:16289537, ECO:0000269|PubMed:25238095, ECO:0000269|PubMed:29224352, ECO:0000269|PubMed:31073693, ECO:0000269|PubMed:32433612}.

    Molecular Weight

    62.0 kDa

    UniProt

    Q8N697
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