NEK3 Protein (AA 1-511) (Strep Tag)

Catalog No. ABIN3119854

Product Details

Target: NEK3 (NIMA related kinase 3 (NEK3))

Protein Type: Recombinant

Protein Characteristics: AA 1-511

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Purification tag / Conjugate: This NEK3 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Brand: AliCE®

Sequence: MDNYTVLRVI GQGSFGRALL VLQESSNQTF AMKEIRLLKS DTQTSRKEAV LLAKMKHPNI VAFKESFEAE GYLYIVMEYC DGGDLMQRIK QQKGKLFPED TILNWFIQIC LGVNHIHKRR VLHRDIKSKN VFLTHNGKVK LGDFGSARLL SSPMAFACTY VGTPYYVPPE IWENLPYNNK SDIWSLGCIL YELCALKHPF QANSWKNLIL KICQGPIHPL PALYSCKLQG LVKQMLKRNP SHRPSATTLL CRGSLAPLVP KCLPPQIIRE YGEQILDEIK ISTPKNMKKQ DSNRVRRALG EANSASMQEE ERGRKCSHTE LESTGTTPAG NALERAARGN PESGNPQEHG RHTSPASPHR PWWERHGPSS NVEALEKASI LTSSFTAEDD RGGSVIKYEE NARRQWVREP PEALLSMLKD ADLSQAFQTY TIYRPGAEGF LKGPLSEDTA SDSVDGDLDS VMLDPERFEP RLDEEDTDFE EDNENPDWVS ELKKHVGYGD GPGGQLLGER A
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).

Purity: > 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: NEK3 (NIMA related kinase 3 (NEK3))

Alternative Name: Nek3 (NEK3 Products)

Background: Serine/threonine-protein kinase Nek3 (EC 2.7.11.1) (Never in mitosis A-related kinase 3) (NimA-related protein kinase 3),FUNCTION: Protein kinase which influences neuronal morphogenesis and polarity through effects on microtubules. Regulates microtubule acetylation in neurons. Contributes to prolactin-mediated phosphorylation of PXN and VAV2. {ECO:0000269|PubMed:19509051}.

Molecular Weight: 57.5 kDa

UniProt: Q9R0A5