SKP2 Protein (AA 1-424) (Strep Tag)

Catalog No. ABIN3120254

Product Details

Target: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Protein Type: Recombinant

Protein Characteristics: AA 1-424

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Purification tag / Conjugate: This SKP2 protein is labelled with Strep Tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA

Brand: AliCE®

Sequence: MHRKHLQEIP DQSGNVTTSF TWGWDSSKTS ELLSGMGVSA LEKEEVDSEN IPHGLLSNLG HPQSPPRKRV KGKGSDKDFV IIRRPKLSRE NFPGVSWDSL PDELLLGIFS CLCLPELLRV SGVCKRWYRL SLDESLWQSL DLAGKNLHPD VTVRLLSRGV VAFRCPRSFM EQPLGESFSS FRVQHMDLSN SVINVSNLHK ILSECSKLQN LSLEGLQLSD PIVKTLAQNE NLVRLNLCGC SGFSESAVAT LLSSCSRLDE LNLSWCFDFT EKHVQAAVAH LPNTITQLNL SGYRKNLQKT DLCTIIKRCP NLIRLDLSDS IMLKNDCFPE FFQLNYLQHL SLSRCYDIIP DTLLELGEIP TLKTLQVFGI VPEGTLQLLR EALPRLQINC AYFTTIARPT MDSKKNLEIW GIKCRLTLQK PSCL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).

Purity: > 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Alternative Name: Skp2 (SKP2 Products)

Background: S-phase kinase-associated protein 2 (Cyclin-A/CDK2-associated protein p45) (F-box protein Skp2) (F-box/WD-40 protein 1) (FWD1),FUNCTION: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription (By similarity). Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition (PubMed:10790373). Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, FOXO1, UBP43, YTHDF2, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. Promotes ubiquitination and destruction of CDH1 in a CK1-dependent manner, thereby regulating cell migration (By similarity). {ECO:0000250|UniProtKB:Q13309, ECO:0000269|PubMed:10790373}., FUNCTION: Through the ubiquitin-mediated proteasomal degradation of viral proteins may have an antiviral activity. {ECO:0000250|UniProtKB:Q13309}.

Molecular Weight: 47.8 kDa

UniProt: Q9Z0Z3

Pathways: Mitotic G1-G1/S Phases