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EIF2S1 Protein (AA 1-315) (Strep Tag)

EIF2S1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC). SDS, WB, ELISA
Catalog No. ABIN3123918
  • Target See all EIF2S1 Proteins
    EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-315
    Origin
    • 8
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Source
    • 6
    • 4
    • 1
    • 1
    • 1
    Tobacco (Nicotiana tabacum)
    Purification tag / Conjugate
    This EIF2S1 protein is labelled with Strep Tag.
    Application
    SDS-PAGE (SDS), Western Blotting (WB), ELISA
    Sequence
    MPGLSCRFYQ HKFPEVEDVV MVNVRSIAEM GAYVSLLEYN NIEGMILLSE LSRRRIRSIN KLIRIGRNEC VVVIRVDKEK GYIDLSKRRV SPEEAIKCED KFTKSKTVYS ILRHVAEVLE YTKDEQLESL FQRTAWVFDD KYKRPGYGAY DAFKHAVSDP SILDSLDLNE DEREVLINNI NRRLTPQAVK IRADIEVACY GYEGIDAVKE ALRAGLNCST ETMPIKINLI APPRYVMTTT TLERTEGLSV LNQAMAVIKE KIEEKRGVFN VQMEPKVVTD TDETELARQL ERLERENAEV DGDDDAEEME AKAED
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    Characteristics
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified in one-step affinity chromatography
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    Purification
    One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).
    Purity
    > 80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
    Top Product
    Discover our top product EIF2S1 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))
    Alternative Name
    Eif2s1 (EIF2S1 Products)
    Synonyms
    EIF-2 Protein, EIF-2A Protein, EIF-2alpha Protein, EIF2 Protein, EIF2A Protein, Eif2s1 Protein, eif2s1l Protein, fb36a08 Protein, wu:fb36a08 Protein, zgc:56510 Protein, 0910001O23Rik Protein, 2410026C18Rik Protein, 35kDa Protein, Eif2a Protein, eIF2alpha Protein, eif2a Protein, CG9946 Protein, Dmel\\CG9946 Protein, Eif2alpha Protein, IF2A_DROME Protein, M(1)14C Protein, M(1)19-153 Protein, M(1)8e3-10 Protein, deIF2alpha Protein, eIF-2 Protein, eIF-2 a Protein, eIF-2 alpha Protein, eIF2 alpha Protein, eIF2-alpha Protein, eIF2a Protein, eiF-2alpha Protein, elF2alpha Protein, l(1)14Cf Protein, l(1)19-153 Protein, eukaryotic translation initiation factor 2 subunit alpha Protein, eukaryotic translation initiation factor 2, subunit 1 alpha a Protein, eukaryotic translation initiation factor 2, subunit 1 alpha Protein, eukaryotic translation initiation factor 2 alpha subunit Protein, Eukaryotic translation initiation factor 2 subunit 1 Protein, eukaryotic translation initiation factor 2 subunit alpha L homeolog Protein, eukaryotic translation Initiation Factor 2alpha Protein, EIF2S1 Protein, eif2s1a Protein, Eif2s1 Protein, eif2s1 Protein, LOC693063 Protein, CNI00230 Protein, THAPSDRAFT_105 Protein, if2a Protein, eif2s1.L Protein, eIF-2alpha Protein
    Background
    Eukaryotic translation initiation factor 2 subunit 1 (Eukaryotic translation initiation factor 2 subunit alpha) (eIF-2-alpha) (eIF-2A) (eIF-2alpha) (eIF2-alpha),FUNCTION: Member of the eIF2 complex that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA (PubMed:15277680, PubMed:19131336). This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex (PubMed:15277680, PubMed:19131336). Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF2 and release of an eIF2-GDP binary complex (PubMed:15277680, PubMed:19131336). In order for eIF2 to recycle and catalyze another round of initiation, the GDP bound to eIF2 must exchange with GTP by way of a reaction catalyzed by eIF2B (PubMed:15277680, PubMed:19131336). EIF2S1/eIF2-alpha is a key component of the integrated stress response (ISR), required for adaptation to various stress: phosphorylation by metabolic-stress sensing protein kinases (EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2) in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to a attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activators ATF4 and QRICH1, and hence allowing ATF4- and QRICH1-mediated reprogramming (PubMed:15277680, PubMed:21285359). {ECO:0000269|PubMed:15277680, ECO:0000269|PubMed:19131336, ECO:0000269|PubMed:21285359}.
    Molecular Weight
    36.1 kDa
    UniProt
    Q6ZWX6
    Pathways
    Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C
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