EIF2S1 Protein (His tag)
-
- Target See all EIF2S1 Proteins
- EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))
- Protein Type
- Recombinant
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This EIF2S1 protein is labelled with His tag.
- Application
- Western Blotting (WB)
- Supplier Product No.
- 009-001-r86s
- Supplier
- Rockland
- Purpose
- EIF2S1 recombinant protein-HIS Epitope
- Purification
- Recombinant full length human EIF2S1 was expressed in E. coli cells using an N-Terminal his epitope. The purity was determined to be >90% by densitometry.
- Purity
- >90%
- Top Product
- Discover our top product EIF2S1 Protein
-
Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1) (AA 1-315) protein (His tag)
custom-made EIF2S1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1) (AA 1-315) protein (GST tag)EIF2S1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1) (AA 1-315) protein (His tag)EIF2S1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 85 % by SDS - PAGE SDS
Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1) (AA 1-315) protein (His tag)custom-made EIF2S1 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1) protein (Myc-DYKDDDDK Tag)EIF2S1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- Application Notes
-
Western_Blot_Dilution: User Optimized
Other: Kinase Assay-User Optimized
Application_Note: EIF2S1 Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~ 40 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 0.2 μg/μL
- Buffer
- EIF2S1 Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol.
- Storage
- -80 °C
- Storage Comment
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- Expiry Date
- 12 months
-
- Target
- EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))
- Alternative Name
- EIF2S1 (EIF2S1 Products)
- Background
-
Synonyms: EIF-2, EIF-2A, EIF-2alpha, EIF2, EIF2A, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit alpha, eIF-2-alpha, eIF-2A, eIF-2alpha
Background: EIF2S1, also known as eukaryotic translation initiation factor 2, subunit 1 alpha, is a 35 kDa protein in which The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation and promotes the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP (1). The Ser51 in the mature human EIF2-alpha protein acts as the sole site of phosphorylation that leads to repression of protein synthesis. EIF2-alpha also plays an essential role in erythropoiesis (2). EIF2S1 Protein is ideal for investigators involved in Signaling Reagents, Protein Substrates, Angiogenesis, Cellular Stress, Inflammation, Neurobiology, and Ser/Thr Kinases research.
- NCBI Accession
- NM_004094
- Pathways
- Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C
-
