NOXO1 Protein (AA 1-349) (Strep Tag)

Catalog No. ABIN3126940

Product Details

Target: NOXO1 (NADPH Oxidase Organizer 1 (NOXO1))

Protein Type: Recombinant

Protein Characteristics: AA 1-349

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Purification tag / Conjugate: This NOXO1 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MASPRHPVSA HAVALVQMDR LQTFAFSVCW SDNSDTFVRR SWDEFRQLQK TLKKTFPVEA GLLRRSEQVL PKLPDAPLLT RRGHTGRGLV RLRLLDTYVQ ALLATSEHIL RSSALHGFFV PKPLDLEPML PPGSLVILPT PEEPLSQPRG SLDIHSLEAQ SIPCVQPFHT LDIRDRPFHT KAQEILDILL RHPSGWWLVE NKDQQVAWFP APYLEEVATC QGQESGLALQ GSGRQFCTTQ AYEGSRSDEL SVPSGARVHV LETSDRGWWL CRYNGRTGLL PAMSLQPEGL GSLLGRPGFP DSAGADKVAE DRTIPPVVPT RPCMSAIQSR CCSITRRALG QEQGTRVPR
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: NOXO1 (NADPH Oxidase Organizer 1 (NOXO1))

Alternative Name: Noxo1 (NOXO1 Products)

Background: NADPH oxidase organizer 1 (Nox organizer 1) (Sorting nexin-28),FUNCTION: Constitutively potentiates the superoxide-generating activity of NOX1 and NOX3 and is required for the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity. Isoform 3 is more potent than isoform 1 in activating NOX3. Together with NOXA1, may also substitute to NCF1/p47phox and NCF2/p67phox in supporting the phagocyte NOX2/gp91phox superoxide-generating activity. {ECO:0000269|PubMed:12473664, ECO:0000269|PubMed:16431374}.

Molecular Weight: 38.8 kDa

UniProt: Q8VCM2