TTC5 Protein (AA 1-440) (Strep Tag)

Catalog No. ABIN3127985

Product Details

Target: TTC5 (Tetratricopeptide Repeat Domain 5 (TTC5))

Protein Type: Recombinant

Protein Characteristics: AA 1-440

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Purification tag / Conjugate: This TTC5 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MMADEEEEAK HVLQKLQGLV DRLYCFRDSY FETHSVEDAG RKQQDVQEEM EKTLQQMEEV LGSAQVEAQA LMLKGKALNV TPDYSPEAEV LLSKAVKLEP ELVEAWNQLG EVYWKKGDVA SAHTCFSGAL THCKNKVSLQ NLSMVLRQLQ TDSGDEHSRH VMDSVRQAKL AVQMDVLDGR SWYILGNAYL SLYFNTGQNP KISQQALSAY AQAEKVDRKA SSNPDLHLNR ATLHKYEESY GEALEGFSQA AALDPVWPEP QQREQQLLEF LSRLTSLLES KGKTKPKKLQ SMLGSLRPAH LGPCGDGRYQ SASGQKMTLE LKPLSTLQPG VNSGTVVLGK VVFSLTTEEK VPFTFGLVDS DGPCYAVMVY NVVQSWGVLI GDSVAIPEPN LRHHQIRHKG KDYSFSSVRV ETPLLLVVNG KPQNSSSQAS ATVASRPQCE
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: TTC5 (Tetratricopeptide Repeat Domain 5 (TTC5))

Alternative Name: Ttc5 (TTC5 Products)

Background: Tetratricopeptide repeat protein 5 (TPR repeat protein 5) (Stress-responsive activator of p300) (Protein Strap),FUNCTION: Cofactor involved in the regulation of various cellular mechanisms such as actin regulation, autophagy, chromatin regulation and DNA repair (PubMed:11511361, PubMed:15448695, PubMed:18451878, PubMed:30420355). In physiological conditions, interacts with cofactor JMY in the cytoplasm which prevents JMY's actin nucleation activity and ability to activate the Arp2/3 complex (PubMed:30420355). Acts as a negative regulator of nutrient stress-induced autophagy by inhibiting JMY's interaction with MAP1LC3B, thereby preventing autophagosome formation (PubMed:30420355). Involves in tubulin autoregulation by promoting its degradation in response to excess soluble tubulin (By similarity). To do so, associates with the active ribosome near the ribosome exit tunnel and with nascent tubulin polypeptides early during their translation, triggering tubulin mRNA-targeted degradation (By similarity). Following DNA damage, phosphorylated by DNA damage responsive protein kinases ATM and CHEK2, leading to its nuclear accumulation and stability (PubMed:15448695, PubMed:18833288). Nuclear TTC5/STRAP promotes the assembly of a stress-responsive p53/TP53 coactivator complex, which includes the coactivators JMY and p300, thereby increasing p53/TP53-dependent transcription and apoptosis (PubMed:11511361). Also recruits arginine methyltransferase PRMT5 to p53/TP53 when DNA is damaged, allowing PRMT5 to methylate p53/TP53 (PubMed:19011621). In DNA stress conditions, also prevents p53/TP53 degradation by E3 ubiquitin ligase MDM2 (PubMed:11511361). Upon heat-shock stress, forms a chromatin-associated complex with heat-shock factor 1 HSF1 and p300/EP300 to stimulate heat-shock-responsive transcription, thereby increasing cell survival (PubMed:18451878). Mitochondrial TTC5/STRAP interacts with ATP synthase subunit beta ATP5F1B which decreased ATP synthase activity and lowers mitochondrial ATP production, thereby regulating cellular respiration and mitochondrial-dependent apoptosis (PubMed:25168243). Mitochondrial TTC5/STRAP also regulates p53/TP53-mediated apoptosis (PubMed:25168243). {ECO:0000250|UniProtKB:Q8N0Z6, ECO:0000269|PubMed:11511361, ECO:0000269|PubMed:15448695, ECO:0000269|PubMed:18451878, ECO:0000269|PubMed:18833288, ECO:0000269|PubMed:19011621, ECO:0000269|PubMed:25168243, ECO:0000269|PubMed:30420355}.

Molecular Weight: 48.8 kDa

UniProt: Q99LG4

Pathways: Chromatin Binding