NME7 Protein (AA 1-395) (Strep Tag)

Catalog No. ABIN3130454

Product Details

Target: NME7 (Nucleoside Diphosphate Kinase (NME7))

Protein Type: Recombinant

Protein Characteristics: AA 1-395

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Purification tag / Conjugate: This NME7 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Brand: AliCE®

Sequence: MRACQQGRSS SLVSPYMAPK NQSERFAFIA EWYDPNASLL RRYELLFYPT DGSVEMHDVK NRRTFLKRTK YEDLRLEDLF IGNKVNVFSR QLVLIDYGDQ YTARQLGSRK EKTLALIKPD AVSKAGEIIE MINKSGFTIT KLRMMTLTRK EAADFHVDHH SRPFYNELIQ FITSGPVIAM EILRDDAICE WKRLLGPANS GLSRTDAPGS IRALFGTDGV RNAAHGPDTF ASAAREMELF FPSSGGCGPA NTAKFTNCTC CIIKPHAISE GMLGKILIAI RDACFGMSAI QMFNLDRANV EEFYEVYKGV VSEYNDMVTE LCSGPCVAIE IQQSNPTKTF REFCGPADPE IARHLRPETL RAIFGKTKVQ NAVHCTDLPE DGLLEVQYFF KILDN
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (AliCE®).

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: NME7 (Nucleoside Diphosphate Kinase (NME7))

Alternative Name: Nme7 (NME7 Products)

Background: Nucleoside diphosphate kinase homolog 7 (NDK 7) (NDP kinase homolog 7) (3'-5' exonuclease NME7) (EC 3.1.-.-) (Protein kinase NME7) (EC 2.7.-.-) (nm23-H7),FUNCTION: Possesses an intrinsic kinase activity. Displays 3'-5' exonuclease activity with a preference for single-stranded DNA. Does not seem to have nucleoside diphosphate kinase activity. Microtubule inner protein (MIP) part of the dynein-decorated doublet microtubules (DMTs) in cilia axoneme, which is required for motile cilia beating (PubMed:37295417). Functional component of the gamma-tubulin ring complex, implicated in the regulation of the microtubule-nucleating activity of the gamma-tubulin ring complex in centrosomes, in a kinase activity-dependent manner (By similarity). {ECO:0000250|UniProtKB:Q9Y5B8, ECO:0000269|PubMed:37295417}.

Molecular Weight: 44.4 kDa

UniProt: Q9QXL8