SIPA1 Protein (AA 1-1037) (Strep Tag)

Catalog No. ABIN3134095

Recombinant SIPA1 protein expressed in Cell-free protein synthesis (CFPS).

Quick Overview for SIPA1 Protein (AA 1-1037) (Strep Tag) (ABIN3134095)

Target: SIPA1 (Signal-Induced Proliferation-Associated 1 (SIPA1))

Protein Type: Recombinant

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1037

Purification tag / Conjugate: This SIPA1 protein is labelled with Strep Tag.

Sequence: MWAGGVGSPR RGMAPAPTDD LFARKLRQPA RPPLTPNTFE PRPARGPLLR SGSDAGEVRP PTPASPRARA HSHEDASRPA ATPTRLFTDP LALLGLPAEE PEPTFPPVLE PRWFAHYDVQ SLLFDWAPRP RGTGSHTEAN SGTLAEGQTT TSDLLLGAPG FVSELGGEGE LGLGGPISPP VPPALPNAAV SVLEEPQTRT TTYSLEHADL GAGYYRKYFY GKEHQNFFGL DEALGPVAVS LRREEKEGSG GGTLHSYRVI VRTTQLRTLR GTISEDALPP GPPSVSPRKL LEHVAPRLSP TCLRLGSASP KVPRQLLTLD EQVLSFQRKG GILYCRAGQG SEEEMYNNQE AGAAFMQFLT LLGDVVRLKG FESYRAQLDT KTDSTGTHSL YTTYQDHEIM FHVSTMLPYT PNNQQQLLRK RHIGNDIVTI VFQEPGSKPF CPTTIRSHFQ HVFLVVRAHA PCTPHTSYRV AVSRTQDTPA FGPALPEGGG PFAANADFRA FLLAKALNGE QAAGHARQFH AMATRTRQQY LQDLATNEVT TTSLDSASRF GLPSLGGRRR ATPRSPGADV QAAGALMWGV RAAPGARVAA GAETSGPDDA EVPCLLGISA ETLVLVAPRD GRVVFNCACR DVLAWTFSEH QLDLYHGRGE AITLRLDGAP GQAVGEVVAR LQLVSRGCET RELALPRDGQ GRLGFEVDAE GFITHVERFT FAETTGLRPG ARLLRVCGQT LPKLGPETAA QMLRSAPKVC VTVLPPDESG RPRRSFSELY MLSLKEPSRR GGPEPVQDET GKLVILPPTK QLLHFCLKDS SSPPGPGDLT EERTEFLRTH NSLSSGSSLS DEAPVLPNTT PDLLLVTTAN PSAPGTDRET PPSQDQSGSP SSHEDTSDSG PELRASILPR TLSLRNSISK IMSEAGSETL EDEWQSISEI ASTCNTILES LSREGQPISE SGDPKEALKC DSEPEPGSLS EKVSHLESML WKLQEDLQRE KADRAALEEE VRSLRHNNQR LLAESESAAT RLLLASKHLG APTTDLA
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: SIPA1 (Signal-Induced Proliferation-Associated 1 (SIPA1))

Alternative Name: Sipa1

Background: Signal-induced proliferation-associated protein 1 (Sipa-1) (GTPase-activating protein Spa-1),FUNCTION: GTPase activator for the nuclear Ras-related regulatory proteins Rap1, Rsr1 and Ran in vitro, converting them to the putatively inactive GDP-bound state. Affects cell cycle progression. {ECO:0000269|PubMed:7799964}.

Molecular Weight: 112.1 kDa

UniProt: P46062

Pathways: Response to Water Deprivation