MYO3B Protein (AA 1-1305) (Strep Tag)

Catalog No. ABIN3134927

This Recombinant MYO3B protein is produced in Cell-free protein synthesis (CFPS).

Quick Overview for MYO3B Protein (AA 1-1305) (Strep Tag) (ABIN3134927)

Target: MYO3B (Myosin IIIB (MYO3B))

Protein Type: Recombinant

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Application: SDS-PAGE (SDS), ELISA, Western Blotting (WB)

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1305

Purification tag / Conjugate: This MYO3B protein is labelled with Strep Tag.

Sequence: MMLGLESLPD PMETWEIIET IGKGTYGKVY KVANKRDGSL AAVKVLDPVS DMDEEIEAEY NILQFLPSHP NVVKFYGMFY KADRCVGGQL WLVLELCNGG SVTELVKGLL RCGKRLDEAV ISYILYGALL GLQHLHCHRI IHRDVKGNNI LLTTEGGVKL VDFGVSAQLT STRLRRNTSV GTPFWMAPEV IACEQQYDSS YDARCDVWSL GITAIELGDG DPPLFEMHPV KMLFKIPRNP PPTLLHPDSW CEEFNHFISQ CLIKDFEKRP SVTHLLDHPF IKGTQGKVLC LQKQLAKVLQ DQKHRNPVAK TRHERMHTGR PHRVEDAGKC CLEDDLVNLE VLDEDTIIYW LQKRYADALI YTYVGDILIA LNPFQNLSIY SPQFSRLYHG VKRSSNPPHI FASADNAYQC LVTFSKDQCI VISGESGSGK TESAHLIVQH LTFLGKADNQ TLRQKILQVN SLVEAFGNAR TAINDNSSRF GKYLEMMFTP TGAVMGARIS EYLLEKSRVI QQAAGEKNFH IFYYIYAGLY HQKKLAEFRL PEEKPPRYIA GETERVMQDI TSKESYRTQF EAIQHCFKII GFADKEVHSV YRILAGILNI GSIEFAAISS QHQTDKSEVP NPEALENAAC VLCISSEELQ EALTSHCVVT RGETIVRANT VDRAEDVRDA MSKALYGRLF SWIVNRINTL LQPDKNICSA EDRMNVGILD IFGFEDFQRN SFEQLCINIA NEQIQYYFNQ HVFALEQMEY KNEGVDAVLV QYEDNRPLLD MFLQKPLGLL ALLDEESRFP QGTDQTLVDK FEDNLRCKFF WRPKGVELCF GIQHYAGPVL YDASGVLEKN RDTLPADVVV VLRTSENKLL QQLFSIPLTK TGNLAQTRAK ITASSRSLPP HFSAGRAKVD TLEVIRHPEE TTNMKRQTMA SYFRYSLMDL LSKMVVGQPH FIRCIKPNDD RKALQFSQDR VLAQLRSTGI LETVSIRRQG YSHRIFFEEF VKRYYYLAFR AHQTPPANKE SCVAILEKSR LDHWVLGKTK VFLKYYHVEQ LNLLLREVMG RVVMLQAYTK GWLGARRYKR AKEKREKGAI TIQSAWRGYD ARRKLKQRSR RRSESEAHIH TVLQTTPDQK YCPDSGGESN RGHEETSRNC PAEADTDGHP QAQSPPTGCD VTSGHADTAA GYTVAELSVA GTDVSPSLVY HTASAHQRLS PCEDSLKPGS EEGLSQKQRA PRRRCQQPKM LSSPEDTMYY NQLNGTLEYQ GSQRKPRKLG QIKVLDGEDQ YYKCLSPGAC APEETHSVHP FFFSSSPRED PFAQH
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: MYO3B (Myosin IIIB (MYO3B))

Alternative Name: Myo3b

Background: Myosin-IIIb (EC 2.7.11.1),FUNCTION: Probable actin-based motor with a protein kinase activity (By similarity). Required for normal cochlear hair bundle development and hearing. Plays an important role in the early steps of cochlear hair bundle morphogenesis. Influences the number and lengths of stereocilia to be produced and limits the growth of microvilli within the forming auditory hair bundles thereby contributing to the architecture of the hair bundle, including its staircase pattern (PubMed:26754646). Involved in the elongation of actin in stereocilia tips by transporting the actin regulatory factor ESPN to the plus ends of actin filaments (PubMed:22264607). {ECO:0000250|UniProtKB:Q8WXR4, ECO:0000269|PubMed:22264607, ECO:0000269|PubMed:26754646}.

Molecular Weight: 148.1 kDa

UniProt: Q1EG27