ARHGAP17 Protein (AA 1-846) (Strep Tag)

Catalog No. ABIN3135056

This Recombinant ARHGAP17 protein is produced in Cell-free protein synthesis (CFPS).

Quick Overview for ARHGAP17 Protein (AA 1-846) (Strep Tag) (ABIN3135056)

Target: ARHGAP17 (rho GTPase Activating Protein 17 (ARHGAP17))

Protein Type: Recombinant

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Product Details

Protein Characteristics: AA 1-846

Purification tag / Conjugate: This ARHGAP17 protein is labelled with Strep Tag.

Sequence: MKKQFNRMKQ LANQTVGRAE KTEVLSEDLL QIERRLDTVR SMCHHSHKRL IACFQGQHGT DAERRHKKLP LTALAQNMQE ASAQLEESLL GKMLETCGDA ENQLALELSQ HEVFVEKEIM DPLYGIAEVE IPNIQKQRKQ LARLVLDWDS VRARWNQAHK SSGTNFQGLP SKIDTLKEEM DEAGNKVEQC KDQLAADMYN FMAKEGEYGK FFVTLLEAQA DYHRKALAVL EKALPEMRAH QDKWAEKPAF GTPLEEHLKR SGREIALPIE ACVMLLLETG MKEEGLFRIG AGASKLKKLK AALDCSTSHL DEFYSDPHAV AGALKSYLRE LPEPLMTFSL YEEWTQVASV QDQDKKLQYL WTTCQKLPPQ NFVNFRYLIK FLAKLAQTSD VNKMTPSNIA IVLGPNLLWA KQEGTLAEIA AATSVHVVAV IEPIIQHADW FFPGEVEFNV SEAFVPLATP NSNHSSHTGN DSDSGTLERK RPASMAVMEG DLVKKESFGV KLMDFQAHRR GGTLNRKHIA PAFQPPLPPT DGNALAPAGP EPPSQSSRAD SSSGGGPVFS STGILEQGLS PGDSSPPKPK DSVSAAVPAA GRNSNQMTTV PNQAQTGGNS HQLSVSTPHS AAGPSPHTLR RAVKKPAPAP PKPGNLPPGH PGGQSSPGTG TSPKPSARSP SPPQQQQQQQ QQQQQQQQQQ TPGMRRCSSS LPPIQAPSHP PPQPPTQPRL GEQGPEPGPT PPQTPTPPST PPLAKQNPSQ SETTQLHGTL PRPRPVPKPR NRPSVPPPPH PPGTHTVDGG LTSSVPTASR IVTDTNSRVS ESLRSIFPEI HSDLASKEVP GHILLDIDND TESTAL
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: ARHGAP17 (rho GTPase Activating Protein 17 (ARHGAP17))

Alternative Name: Arhgap17

Background: Rho GTPase-activating protein 17 (Neuron-associated developmentally-regulated protein) (Nadrin) (Rho-type GTPase-activating protein 17),FUNCTION: Rho GTPase-activating protein involved in the maintenance of tight junction by regulating the activity of CDC42, thereby playing a central role in apical polarity of epithelial cells. Specifically acts as a GTPase activator for the CDC42 GTPase by converting it to an inactive GDP-bound state. The complex formed with AMOT acts by regulating the uptake of polarity proteins at tight junctions, possibly by deciding whether tight junction transmembrane proteins are recycled back to the plasma membrane or sent elsewhere. Participates in the Ca(2+)-dependent regulation of exocytosis, possibly by catalyzing GTPase activity of Rho family proteins and by inducing the reorganization of the cortical actin filaments. Acts as a GTPase activator in vitro for RAC1 (By similarity). {ECO:0000250}.

Molecular Weight: 92.2 kDa

UniProt: Q3UIA2