REXO1 Protein (AA 1-1213) (Strep Tag)

Catalog No. ABIN3135965

Product Details

Target: REXO1 (REX1, RNA Exonuclease 1 Homolog (REXO1))

Protein Type: Recombinant

Protein Characteristics: AA 1-1213

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Purification tag / Conjugate: This REXO1 protein is labelled with Strep Tag.

Application: SDS-PAGE (SDS), ELISA, Western Blotting (WB)

Sequence: MLRSTGFFRS IDCPYWAGAP GGPCRRPYCH FRHRGARGPG APGSGGAASP ASGLGYDPYN PELPQPPVQR ENGALGQGDG MLELELVNQA IEAVRGEVEL EQRRYQELLE TARGHSAGPS ALAPCSPATS IDDDDTFSLA LTYTPGGLLS PDAAYQPTPL AVPAEPGHKY SLAPSDRSQG RAAGGAGALE YVPKAVGQPR RCGRPVSGGK YVVDSSKPST DLEYDPLSNY SARHLGRASA RDERATKRPR GSRGAEPYTP ALKKPCDPFG GCEARFSDSE DDVTSPPKAD VSSPKAGADP ESKAPGKPVS KEGREHEDGG LRGTKEMAVQ YDVEDLGQPP KAPDTVSVVK PGSPARASQD ARVPKEGKAK KKKSGTSASL SHKDKVRKKD KKKEKDPARP RGKEKVCTDK KKLPASNPRG KAQGPEGTKK KPSSATTVAS SGKGGSGRPS STGPQDSGPG PHAPLAWKAG SAKKMSSGKL VERKARSLDE GAPQDTPKLK KRALSHAELF GDESEEEDSS LGAGAPRVWP PTLPSLSSDS ESDSDSSLGL DETKVPKRLK AAPPASPVPP SPLSSSSSSS GASQCAEEDV DYSALEKEVD FDVDPMEECL RIFNESTSVK TEDKGRLARQ PPKEKAEEKT HAGLTTLFPG QKRRVSHLCK PGKESEAPKR TPVAPPARPP TAQEVCYRRA QLAQRDSASW LQAAPRPTER LSSVHISAPG EKRRIAHVPN PRLAAAPTGA KRALSASSSQ ASHGPEPGSQ PLKTRTLSGM ASKTTTTVTP KRIAHSPSLQ SLKKPVIPKE FGGKVPTVVR QRYLNLFIEE CLKFCSSNQE AIEKALNEEK VAYDRSPSKN IYLNVAVNTL KKLRGLVPNT VPNLSKASGR RVVSHEVVLG GKLAAKTSFS LSRPSSPRVE ELKGTALYSR LREYLLTQEQ LKENGYPFPH PERPGGAIIF TAEEKKPKDP SCRICCRCGT EYLVSSSGRC VRSEECYYHW GRLRRNRVAG GWETQYMCCS AAVGSVGCQV AKQHVQDGRK ENLEGFVRTF QKELPEDAHA GVFALDCEMS YTTYGLELTR VTVVDTDMQV VYDTFVKPDN EVVDYNTRFS GVTEADLVDT SITLRDVQAV LLSMFSADTI LIGHSLESDL LALKVIHGTV VDTSVLFPHR LGLPYKRSLR NLMADYLRQI IQDNVDGHSS SEDASACMHL VIWKIREDAK TKR
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: REXO1 (REX1, RNA Exonuclease 1 Homolog (REXO1))

Alternative Name: Rexo1 (REXO1 Products)

Background: RNA exonuclease 1 homolog (EC 3.1.-.-) (Transcription elongation factor B polypeptide 3-binding protein 1),FUNCTION: Seems to have no detectable effect on transcription elongation in vitro. {ECO:0000250}.

Molecular Weight: 130.8 kDa

UniProt: Q7TT28