RAB11FIP3 Protein (AA 1-1047) (Strep Tag)

Catalog No. ABIN3136469

Recombinant RAB11FIP3 protein expressed in Cell-free protein synthesis (CFPS).

Quick Overview for RAB11FIP3 Protein (AA 1-1047) (Strep Tag) (ABIN3136469)

Target: RAB11FIP3 (RAB11 Family Interacting Protein 3 (Class II) (RAB11FIP3))

Protein Type: Recombinant

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1047

Purification tag / Conjugate: This RAB11FIP3 protein is labelled with Strep Tag.

Sequence: MELCQPTSLS DHDQPASGPQ RGVMGLVGPD APRGWSEEPE EHAQLQRWPE GPNAPICWPE EVEEPHAPSR WAKEPNAPRC SSQEPDESCH LAEELEESDS PRCWPQEPDT PCHLAKELEE PDAPRCLPQE PDTPCYLAKE LEEPNIPRCW PQEPDVPCHL AKELEEPDAP RCWPQEPDAF CHLLKEVEEP DALRCWLQGP DAPCHLAKEL EDLDSPRCWP QEPDESCHLA KELEEPDAPC HLAKELEEPD APRCWPQEPD VPCLLAKKWE ESDAPCLLTE ELEEPDALHC WPQESEAPCL LAKELEEPDA SHSCPQEADT GCLSAKEPEE PDVSHLWQGV PDAPCLLVKE PEEADALHCC WPEESEEPDA LNPPCFWANE PDEPDPSRCW SEEPQVLCLW PEEQNTKRCW QEEPDAPCFW PEDREEPIVS CLQFKEPEKP KVRSSWPEEL EDCCPTRGLP LEPLLADGEL LQACPGPPSD PGPALSLPSE PGTAQEEGAR LRAVFDALDR DGDGFVRIED FIQFATVYGA EQVKDLTQYL DPSGLGVISF EDFYQGIVAI RNGDPDGQLY SVEPVQDEET PACADEFDDF VTYEANEVTD SAYMGSESTY SECETFTDED TSTLVHPELQ PEGDVDSAGG SGVPSECLDT MEEPDHGALL LLPGRSRPHS QAVVMVIGSE EHFEDYGEGN EAELSPETLC DGDGEDPAFL TPSPAKRLSS RKVARYLHQS GTLTMEALED PPPEPVECPE EDIADKVIFL ERRVSELEKD SAAAGEQHGR LRQENLQLVH RANALEEQLK EQEFRAQEKV LEETRKQKEL LCKMEREKSI EIENLQARLQ QLDEENSELR SCTPCLKANI ERLEEEKQKM LDEIEELTQR LSEEQENKRK MGDRLSHERH QFQRDKEATQ ELIEDLRKQL EHLQLLRLEV EQRRGRSSSL GLQEYNSRAR ESELEQEVRR LKQDNRNLKE QNDELNGQII TLSIQGAKSL FSTSFSESLA AEISSVSRDE LMEAIQKQEE INFRLQDYID RIIVAILETN PSILEVK
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: RAB11FIP3 (RAB11 Family Interacting Protein 3 (Class II) (RAB11FIP3))

Alternative Name: Rab11fip3

Background: Rab11 family-interacting protein 3 (FIP3) (FIP3-Rab11) (Rab11-FIP3),FUNCTION: Downstream effector molecule for Rab11 GTPase which is involved in endocytic trafficking, cytokinesis and intracellular ciliogenesis by participating in membrane delivery (PubMed:18685082). Recruited by Rab11 to endosomes where it links Rab11 to dynein motor complex. The functional Rab11-RAB11FIP3-dynein complex regulates the movement of peripheral sorting endosomes (SE) along microtubule tracks toward the microtubule organizing center/centrosome, generating the endocytic recycling compartment (ERC) during interphase of cell cycle. Facilitates the interaction between dynein and dynactin and activates dynein processivity (By similarity). Binding with ASAP1 is needed to regulate the pericentrosomal localization of recycling endosomes (PubMed:18685082). The Rab11-RAB11FIP3 complex is also implicated in the transport during telophase of vesicles derived from recycling endosomes to the cleavage furrow via centrosome-anchored microtubules, where the vesicles function to deliver membrane during late cytokinesis and abscission. The recruitment of Rab11-RAB11FIP3-containing endosomes to the cleavage furrow and tethering to the midbody is co-mediated by RAB11FIP3 interaction with ARF6-exocyst and RACGAP1-MKLP1 tethering complexes. Also involved in the Rab11-Rabin8-Rab8 ciliogenesis cascade by facilitating the orderly assembly of a ciliary targeting complex containing Rab11, ASAP1, Rabin8/RAB3IP, RAB11FIP3 and ARF4, which directs preciliary vesicle trafficking to mother centriole and ciliogenesis initiation. Also promotes the activity of Rab11 and ASAP1 in the ARF4-dependent Golgi-to-cilia transport of the sensory receptor rhodopsin. Competes with WDR44 for binding to Rab11, which controls intracellular ciliogenesis pathway. May play a role in breast cancer cell motility by regulating actin cytoskeleton (By similarity). {ECO:0000250|UniProtKB:O75154, ECO:0000269|PubMed:18685082}.

Molecular Weight: 118.5 kDa

UniProt: Q8CHD8