MTA1 Protein (AA 1-715) (Strep Tag)

Catalog No. ABIN3136603

This Recombinant MTA1 protein is expressed in Cell-free protein synthesis (CFPS).

Quick Overview for MTA1 Protein (AA 1-715) (Strep Tag) (ABIN3136603)

Target: MTA1 (Metastasis Associated 1 (MTA1))

Protein Type: Recombinant

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Product Details

Protein Characteristics: AA 1-715

Purification tag / Conjugate: This MTA1 protein is labelled with Strep Tag.

Sequence: MAANMYRVGD YVYFENSSSN PYLIRRIEEL NKTANGNVEA KVVCFYRRRD ISSSLIALAD KHATLSVCYR AGPGADTGEE GEVEEEVENP EMVDLPEKLK HQLRHRELFL SRQLESLPAT HIRGKCSVTL LNETESLKSY LEREDFFFYS LVYDPQQKTL LADKGEIRVG NRYQADITDL LKEGEEDGRD QSKLETKVWE AHNPLVDKQI DQFLVVARSV GTFARALDCS SSVRQPSLHM SAAAASRDIT LFHAMDTLHK NIYDISKAIS ALVPQGGPVL CRDEMEEWSA SEANLFEEAL EKYGKDFTDI QQDFLPWKSL TSIIEYYYMW KTTDRYVQQK RLKAAEAESK LKQVYIPNYN KPNPNQISAS SVKATVVNGT GTPGQSPGAG RACESCYTTQ SYQWYSWGPP NMQCRLCASC WTYWKKYGGL KMPTRLDGER PGPNRNNMSP HGIPARSSGS PKFAMKTRQA FYLHTTKLTR IARRLCREIL RPWHAARHPY MPINSAAIKA ECTARLPEAS QSPLVLKQVV RKPLEAVLRY LETHPRPPKP DPVKSSSSVL SSLTPAKSAP VINNGSPTIL GKRSYEQHNG VDGNMKKRLL MPSRGLANHG QTRHMGPSRN LLLNGKSYPT KVRLIRGGSL PPVKRRRMNW IDAPDDVFYM ATEETRKIRK LLSSSETKRA ARRPYKPIAL RQSQALPLRP PPPAPVNDEP IVIED
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: MTA1 (Metastasis Associated 1 (MTA1))

Alternative Name: Mta1

Background: Metastasis-associated protein MTA1,FUNCTION: Transcriptional coregulator which can act as both a transcriptional corepressor and coactivator (PubMed:20682799, PubMed:20071335). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (By similarity). In the NuRD complex, regulates transcription of its targets by modifying the acetylation status of the target chromatin and cofactor accessibility to the target DNA (PubMed:17671180). In conjunction with other components of NuRD, acts as a transcriptional corepressor of BRCA1, ESR1, TFF1 and CDKN1A (PubMed:20071335, PubMed:21965678). Acts as a transcriptional coactivator of BCAS3, PAX5 and SUMO2, independent of the NuRD complex (PubMed:17671180, PubMed:21965678). Stimulates the expression of WNT1 by inhibiting the expression of its transcriptional corepressor SIX3 (PubMed:20682799). Regulates p53-dependent and -independent DNA repair processes following genotoxic stress (PubMed:20071335, PubMed:19805145). Regulates the stability and function of p53/TP53 by inhibiting its ubiquitination by COP1 and MDM2 thereby regulating the p53-dependent DNA repair (PubMed:19837670, PubMed:19805145). Plays a role in the regulation of the circadian clock and is essential for the generation and maintenance of circadian rhythms under constant light and for normal entrainment of behavior to light-dark (LD) cycles (PubMed:24089055). Positively regulates the CLOCK-BMAL1 heterodimer mediated transcriptional activation of its own transcription and the transcription of CRY1 (PubMed:24089055). Regulates deacetylation of BMAL1 by regulating SIRT1 expression, resulting in derepressing CRY1-mediated transcription repression (PubMed:24089055). With Tfcp2l1, promotes establishment and maintenance of pluripotency in embryonic stem cells (ESCs) and inhibits endoderm differentiation (PubMed:28982712). {ECO:0000250|UniProtKB:Q13330, ECO:0000269|PubMed:17671180, ECO:0000269|PubMed:19805145, ECO:0000269|PubMed:19837670, ECO:0000269|PubMed:20071335, ECO:0000269|PubMed:20682799, ECO:0000269|PubMed:21965678, ECO:0000269|PubMed:24089055, ECO:0000269|PubMed:28982712}.

Molecular Weight: 80.8 kDa

UniProt: Q8K4B0

Pathways: Chromatin Binding