SYNPO2 Protein (AA 1-1087) (Strep Tag)

Catalog No. ABIN3136833

This Recombinant SYNPO2 protein is produced in Cell-free protein synthesis (CFPS).

Quick Overview for SYNPO2 Protein (AA 1-1087) (Strep Tag) (ABIN3136833)

Target: SYNPO2 (Synaptopodin 2 (SYNPO2))

Protein Type: Recombinant

Origin: Mouse

Source: Cell-free protein synthesis (CFPS)

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Purity: approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade: custom-made

Product Details

Protein Characteristics: AA 1-1087

Purification tag / Conjugate: This SYNPO2 protein is labelled with Strep Tag.

Sequence: MGTGDFICIS MTGGAPWGFR LQGGKEEQQP LQVAKIRSQS KASGSGLREG DEVVSINGNP CADLTYPEVI KLMEGITDSL HLLVKRPSSG TSETLDSESE TTNHQHLTHE GPMESTTLQI QQATETQSED FFLAPVQTKV PLTEDQSNAW GYAECPKEEQ APPMLGSQEG HLVEEVILRQ KAEAGQPGHV VELQLSLSKE RHQCTSGPIV TLQGNDKSTS PDPDWSSQLE RTVHINSIPA PEKADTSLTS STSSGRELRV IQGRDPGGAG LPQVEVILDC SDRLKAEECR LQTGRGCVAS PVEGGRSEAP PSLVSFAVSS EGTEHGEDQR SGKDQSRPHK HRARHARLRR SESLSEKQVK EAKSKCKSIA LLLTDAPNPN SKGVLMFKKR RRRARKYTLV SYGTGELERE EEEEEDQEAG DKDEISEVAF LGTSESEVDE ELLSDVDDNT QVVNFDWDSG LVDIEKRLNR GDKMEMLPDT TGKGALMFAK RRERMEQFTA QNEEEKTGGM AGGGPDALQT DGLRTMTSYQ RKEESVRMQS SVSESSFQMG RSLASVPQQN GFSGVSETAG AQRMFPMNRT AKPFLGSMNQ PAAPFSPTRS VTSPISDFPA PPPYSAVSPP PEAFSRGVSS PVAGPAQPPP WPQPAPWSQP AFYDSSEQIA SRDERIAVPA KRTGILQEAK RRGTTKPMFT FKETKVSPNP ELLSLLQNAE GKRGTGGDSG PEEDYLSLGA EACNFMQSSA KQKTPPPVAP KPAVKSPSSS QPVAPVSPVW SPGVAPAQRP AFSTSNPPNP PQVTAVSSIK IAQPAAPPAR PASALNLAGP FKGPQAVVVS HNYTPKPSAP TPLVNAAPAG AGGPSNELPG MSGKGAQLFA KRQSRMEKYV VDSDTVQAHT VRAQSPTPSL PASWKYSSNV RAPPPVAYNP IHSPSYPLAA IKSQPPGAQA SKTSKKKGKK PLNTLDVMKH QPYQLNASLF TFQPPDSKDG LPQKSTVKVS SAPAMKQALP PRQANVGSPT NAQASSVYSV PAYTSQPNFF AAEATSPVSA SPVPVSVPTS PKQESTSTSY FVAPRPKFSA KKSGVTVQVW KPSVVEE
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified in one-step affinity chromatography
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: SYNPO2 (Synaptopodin 2 (SYNPO2))

Alternative Name: Synpo2

Background: Synaptopodin-2 (Myopodin),FUNCTION: Has an actin-binding and actin-bundling activity (PubMed:11673475). Can induce the formation of F-actin networks. At the sarcomeric Z lines is proposed to act as adapter protein that links nascent myofibers to the sarcolemma via ZYX and may play a role in early assembly and stabilization of the Z lines. Involved in autophagosome formation. May play a role in chaperone-assisted selective autophagy (CASA) involved in Z lines maintenance in striated muscle under mechanical tension, may link the client-processing CASA chaperone machinery to a membrane-tethering and fusion complex providing autophagosome membranes. Involved in regulation of cell migration. May be a tumor suppressor (By similarity). {ECO:0000250|UniProtKB:D4A702, ECO:0000250|UniProtKB:Q9UMS6, ECO:0000269|PubMed:11673475}.

Molecular Weight: 116.5 kDa

UniProt: Q91YE8